NDLI: Methylation of the APAF-1 and DAPK-1 promoter region correlates with progression of renal cell carcinoma in North Indian population

Content Provider	Springer Nature Link
Author	Ahmad, Shiekh Tanveer Arjumand, Wani Seth, Amlesh Saini, Ashish Kumar Sultana, Sarwat
Copyright Year	2011
Abstract	Aberrant promoter hypermethylation of cancer associated genes occur frequently during carcinogenesis and may serve as a cancer biomarker. The aim of this study was to investigate the occurrence and relevance of promoter methylation of the tumor suppressor DAPK-1, APAF-1 () and SPARC in relation to different pathological stages and histological grades of tumor progression that might act as possible independent prognostic factor in the susceptibility towards renal cell carcinoma (RCC) in North Indian population. Three tumor suppressor gene promoters namely APAF-1, DAPK-1 and SPARC were assessed by methylation-specific PCR (MS-PCR) in 196 primarily resected renal cell tumors paired with the corresponding normal tissue samples. After genomic DNA isolation and sodium bisulfite modification, methylation levels were determined and correlated with standard clinicopathological parameters, pathological stage and Fuhrman nuclear grade of RCC. Significant differences in methylation frequency among the four subtypes of renal tumors were found for APAF-1 (p < 0.001), DAPK-1 (p < 0.001) and SPARC (p = 0.182), when compared with the corresponding normal tissue. Male subjects showed stronger association of methylation frequency of all the three genes with RCC than the female subjects. Additionally, higher frequency of APAF-1, DAPK-1 and SPARC promoter methylation were directly correlated with higher tumor stage (p $^{trend}$ < 0.001). Higher frequency of promoter methylation of APAF-1 and SPARC were also associated with higher nuclear grade (p < 0.001 and p = 0.036, respectively). This gene panel might contribute to a more optimal diagnostic coverage and information, improving preoperative assessment and therapeutic decision-making in patients harboring suspicious renal masses.
Starting Page	395
Ending Page	402
Page Count	8
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	33
Issue Number	2
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-09-16
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	APAF-1 DAPK-1 SPARC Promoter methylation PCR AJCC cancer stage and Fuhrman’s grade Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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