NDLI: Characterization of 20 microsatellite loci by multiplex PCR in swimming crab, Portunus trituberculatus

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Characterization of 20 microsatellite loci by multiplex PCR in swimming crab, Portunus trituberculatus

Content Provider	Springer Nature Link
Author	Lee, Hye Jin Lee, Do Hyun Yoon, Seung Jong Kim, Dae Hyun Kim, Sang Gyu Hyun, Young Se Min, Gi Sik Chung, Ki Wha
Copyright Year	2013
Abstract	The swimming crab, Portunus trituberculatus (Crustacea: Decapoda: Brachyura), is one of the most important fishery resources in East Asia. The aim of this work is to develop microsatellite multiplex PCR systems for 20 microsatellites which were isolated from a microsatellite-enriched genomic library and to explore the genetic diversity of P. trituberculatus captured in the coastal regions of the Yellow Sea in Korea. We prepared four multiplex PCR systems (two hexplex and tetraplex systems each) which could be used for the genotyping of 20 markers. Most markers in this system are highly informative with mean polymorphism information contents and observed heterozygosity of 0.913 and 0.930. The total number of alleles observed in this study was 724, and the mean allelic number per locus was 36.2. Significant deviations from the Hardy–Weinberg equilibrium (HWE) were observed at six loci in the Wonsando Island population, but all the loci deviated from the HWE in the Jeonjangpo population. The genetic differentiation between the two populations and the phylogenetic tree constructed by the unweighted pair group method with the arithmetic average suggested that there is no significant genetic difference between Wonsando Island and Jeonjangpo populations. The prepared multiplex PCR systems and population genetic data will be helpful to study phylogeographic analysis as well as to prepare strategies of stock management and aquaculture of P. trituberculatus.
Starting Page	77
Ending Page	85
Page Count	9
File Format	PDF
ISSN	19769571
Journal	Genes & Genomics
Volume Number	35
Issue Number	1
e-ISSN	20929293
Language	English
Publisher	The Genetics Society of Korea
Publisher Date	2013-01-29
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microsatellite Multiplex PCR Polymorphism Portunus trituberculatus Swimming crab Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology

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