NDLI: Molecular characterization of soybean GmDjp1 encoding a type III J-protein induced by abiotic stress

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Molecular characterization of soybean GmDjp1 encoding a type III J-protein induced by abiotic stress

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Genes & Genomics : Volume 35, Issue 1, February 2013

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Genes & Genomics : Volume 33

Genes & Genomics : Volume 32

Genes & Genomics : Volume 31

Molecular characterization of soybean GmDjp1 encoding a type III J-protein induced by abiotic stress

Content Provider	Springer Nature Link
Author	So, Hyun Ah Chung, Eunsook Lee, Jai Heon
Copyright Year	2013
Abstract	Heat stress severely affects plant growth and development causing crop loss worldwide. Classical type I DnaJ proteins (also called as J-proteins, J-domain proteins or HSP40 proteins) function as molecular co-chaperones for the HSP70 proteins. In this study, we have cloned and characterized a novel gene GmDjp1 (G lycine m ax DnaJ protein 1) encoding a type III J-protein of which function has not been identified in plant. Deduced amino acid sequences of GmDjp1 show the highest homology with a J-protein from Medicago truncatula legume plant (83 %) and with Arabidopsis thaliana type III J-class proteins, atDjC53 (77 %) and atDjC32 (50 %). DNA blot analysis revealed that GmDjp1 exists as a 2-copy gene in soybean genome. GmDjp1 mRNA was induced by a broad spectrum of abiotic stresses, including wounding, heat-shock, dehydration, cold or high-salinity stress, suggesting its role in the signaling events in the abiotic stress-related defense response. Subcellular localization studies demonstrated that the GmDjp1-GFP fusion protein was localized in the nucleus. Differential RNA expression of GmDjp1 by heat-shock stress inspired us to test heat-shock tolerance of GmDjp1in E. coli. Heterologous expression of GmDjp1 conferred tolerance to high temperature stress in E. coli. This report provides strong evidence that GmDjp1 may play a critical role during heat-shock stress in cell.
Starting Page	247
Ending Page	256
Page Count	10
File Format	PDF
ISSN	19769571
Journal	Genes & Genomics
Volume Number	35
Issue Number	2
e-ISSN	20929293
Language	English
Publisher	The Genetics Society of Korea
Publisher Date	2013-01-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress DnaJ GFP Heat-shock Soybean Microbial Genetics and Genomics Plant Genetics & Genomics Animal Genetics and Genomics Human Genetics
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology

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