NDLI: Discovering Transgenic Elite Events: Using Information from Early Screening Trials for Improving Experimental Design

Content Provider	Springer Nature Link
Author	Wolf, J. Duchateau, L. Verbeke, G. Schrevens, E.
Copyright Year	2010
Abstract	The transgenic improvement of a commercial crop requires a suitable transgene as well as a suitable insertion location in the crop’s genome. An elite event is a successful insertion of an active transgene, and many events need to be screened to detect such elite events. This is expensive, hence optimization is needed.The screening of events in a commercial crop is usually preceded by the screening of genes in an easily transformable test plant, either a non-commercial variety of the crop or another species. This first phase mainly aims at discovering active genes. The results from the first phase can also be utilized to predict the probability of finding elite events with similar or larger effects in the commercial crop, making use of estimated variance components in the mixed model framework. The theoretical relationship between these variance components and the probability to discover in the second phase an event with an effect of a predefined magnitude is studied.The consequences of having to estimate the variance components from a first phase experiment are studied through simulations. These show that, in order to be useful to assist in the design of the second phase, the first phase should involve a large number of events, typically more than 30. The number of events is much more important than the number of plants within an event. The approach is applied to real data obtained in a commercial screening facility.
Starting Page	403
Ending Page	415
Page Count	13
File Format	PDF
ISSN	10857117
Journal	Journal of Agricultural, Biological, and Environmental Statistics
Volume Number	15
Issue Number	3
e-ISSN	15372693
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-04-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gene screening Insertion variability Mixed models Somaclonal variance Transgenic crop improvement Variance components Biostatistics Environmental Monitoring/Analysis Agriculture Statistics for Life Sciences, Medicine, Health Sciences
Content Type	Text
Resource Type	Article
Subject	Applied Mathematics Statistics and Probability Environmental Science Agricultural and Biological Sciences Statistics, Probability and Uncertainty

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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