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| Content Provider | Springer Nature Link |
|---|---|
| Author | Bhat, Towseef Mohsin Ansari, Mohd Yunus Khalil Aslam, Rumana |
| Copyright Year | 2012 |
| Abstract | The effect of sodium azide (NaN3) was studied in Psoralea corylifolia L (2n = 22). Three distinct phenotypic variants one at 0.50 % and two at 0.75 % concentrations of NaN$_{3}$ were selected for analysis. Besides morphological variations in the form of size, height, number of branches, number of flowers and seeds, cytological anomalies in the form of pollen fertility, univalents, multivalents, laggards, bridges, micronuclei, were also observed in all variants and the anomalies showed decreasing order at the final stages of both meiosis and mitosis. These variants were further analysed using randomly amplified polymorphic DNA (RAPD) technique, which differentiated the variants genotypically, on the basis of occurrence of polymorphic DNA bands. The percentage of polymorphism was found to be highest (70 %) using primer OPH-12 followed by OPC-02 having 66.66 % of polymorphism. Among 31 DNA bands 19 were found to be polymorphic which showed great variation created by NaN$_{3}$ in the genome of the experimental plant. Therefore the above concentrations of sodium azide are best suited for the induction of genetic variations in Psoralea corylifolia. |
| Starting Page | 149 |
| Ending Page | 154 |
| Page Count | 6 |
| File Format | |
| ISSN | 0029568X |
| Journal | The Nucleus |
| Volume Number | 55 |
| Issue Number | 3 |
| e-ISSN | 09767975 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-12-20 |
| Publisher Place | India |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cytology Mutation Psoralea corylifolia RAPD Sodium azide Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Genetics Molecular Biology Molecular Medicine |
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