NDLI: Liquid crystal-based biosensors using a strong polyelectrolyte-containing block copolymer, poly(4-cyanobiphenyl-4′-oxyundecylacrylate)-b-poly(sodium styrene sulfonate)

Content Provider	Springer Nature Link
Author	Omer, Muhammad Islam, Mohammad Tariqul Khan, Mashooq Kim, Young Kyoo Lee, Joon Hyung Kang, Inn Kyu Park, Soo Young
Copyright Year	2014
Abstract	The interface between a nematic liquid crystal phase, 4-cyano-4′-pentylbiphenyl (5CB) and water was examined for protein detection by monitoring the formation of a complex between sodium polystyrene sulfonate (PSSNa) and a positively charged biological species on the 5CB in a transmission electron microscopy (TEM) grid cell coated with a strong anionic polyelectrolyte-containing block copolymer, LCP-b-PSSNa (LCP:poly(4-cyanobiphenyl-4′-oxyundecylacrylate)). This block copolymer was successfully synthesized by reversible addition-fragmentation chain transfer polymerization. A monolayer of LCP-b-PSSNa in a Langmuir Blodgett trough (in which PSSNa and LCP were located in and above water, respectively, in the TEM grid cell) was transferred to the 5CB/water interface in the 5CB-filled TEM grid that was already placed on octadecyltrichlorosilane-coated glass. Model proteins such as bovine serum albumin (BSA), hemoglobin (Hb), α chymotrypsinogen-A (ChTg), and lysozyme (LYZ) having different isoelectric points (pIs) were tested for non-specific protein detection. When the protein solutions were injected into the TEM grid cell, the initial homeotropic orientation of 5CB in the TEM grid cell changed to a planar one below the pIs of the proteins due to electrostatic interactions between PSSNa (- charge) and the proteins (+ charge); this did not occur above the pIs of the tested proteins. The minimum concentrations at which the homeotropic to planar configurational changes (H-P changes) occurred were 0.02, 0.04, 0.04, and 0.08 wt% for BSA, Hb, ChTg, and LYZ, respectively. Therefore, the positively charged biomaterials were visually detected at the PSSNa-coated LC/water interface during an H-P change by using polarized optical microscopy under crossed polarizers. This simple set-up for non-specific biomaterial detection paves a way for the development of efficient and excellent quality biosensors.
Starting Page	888
Ending Page	894
Page Count	7
File Format	PDF
ISSN	15985032
Journal	Macromolecular Research
Volume Number	22
Issue Number	8
e-ISSN	20927673
Language	English
Publisher	The Polymer Society of Korea
Publisher Date	2014-08-01
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	strong anionic polyelectrolyte poly(4-cyanobiphenyl-4′-oxyundecylacrylate)-b-poly(sodium styrene sulfonate) monolayer protein reversible addition-fragmentation polymerization biosensor Polymer Sciences Soft and Granular Matter, Complex Fluids and Microfluidics Physical Chemistry Characterization and Evaluation of Materials Nanochemistry Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Materials Chemistry Chemical Engineering Polymers and Plastics

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