NDLI: Synthetic optimization of gelatin-oleic conjugate and aqueous-based formation of self-assembled nanoparticles without cross-linkers

Content Provider	Springer Nature Link
Author	Nguyen, Van Hong Lee, Beom Jin
Copyright Year	2017
Abstract	Amphiphilically-modified gelatin has the ability to form self-assembled NPs which own hydrophobic inner core for water-insoluble drugs loading and gelatin layer for circulation extension and biocompatibility. Gelatin-oleic acid conjugates (GOC) was synthesized from oleic acid (OA) and gelatin, using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) in 60% ethanol. The effect of various gelatin concentrations, oleic acid-gelatin molar ratio and reaction time on the substitution of GOC was evaluated. Fourier transform infrared (FT-IR) spectroscopy and proton nuclear magnetic resonance ($^{1}$H NMR) revealed the successful synthesis of GOC. The ratio of oleic acid-reacted amino groups within gelatin was above 50% as quantified using the 2,4,6-trinitrobenzene sulfonic acid (TNBS) method. Based on the physicochemical changes of GOC upon the modification of various synthesis factors, the optimized process for the GOC synthesis was well established. Self-assembled gelatin-oleic acid nanoparticles (GON) were fabricated simply by the desolvation method using dialysis membrane, without adding a cross-linker. GON was formed at a lower critical micelle concentration (CMC) in water, 0.3 mg/mL. GON has a homogenous size of 100-300 nm, which was showed in the electrophoretic light scattering (ELS) results, scanning electron microscope (SEM) and transmission electron microscope (TEM) images. The zeta potential of GON indicated a slightly negative surface charge (-6 mV). The GON showed no toxicity in human embryonic kidney cells (HEK 293) and significantly improved coumarin loading in adenocarcinomic human alveolar basal epithelial cells (A549). The current GOC and GON could provide versatile strategies to deliver cargo into the body by acting as a safe and biocompatible nanoparticulate carrier.
Starting Page	466
Ending Page	473
Page Count	8
File Format	PDF
ISSN	15985032
Journal	Macromolecular Research
Volume Number	25
Issue Number	5
e-ISSN	20927673
Language	English
Publisher	The Polymer Society of Korea
Publisher Date	2017-06-03
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	gelatin-oleic acid conjugate aqueous-based self-assembled nanoparticles physicochemical properties synthetic optimization cellular uptake Polymer Sciences Soft and Granular Matter, Complex Fluids and Microfluidics Physical Chemistry Characterization and Evaluation of Materials Nanochemistry Nanotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Materials Chemistry Chemical Engineering Polymers and Plastics

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