NDLI: Fabrication of selective anti-biofouling surface for micro/nanopatterning of proteins

Content Provider	Springer Nature Link
Author	Jeong, Heon Ho Lee, Ji Hye Lee, Chang Soo Jang, Hongchul Yang, Yung Hun Kim, Yon Hwan Huh, Kang Moo
Copyright Year	2010
Abstract	This paper reports a simple method for creating a functionalized surface for the efficient micro/nanopatterning of proteins by micromolding in capillaries (MIMIC) of poly(ethylene glycol)-poly(lactide) diblock copolymer (PEG-PLA) and self-assembled polyelectrolyte multilayers (PEL). The fabricated surface consisted of two distinct regions: a PEL region to promote protein immobilization and a PEG-PLA background as a biological barrier to prevent the nonspecific binding of proteins. When the ability of anti-biofouling of PEG-PLA was compared with the most widely used blocking agents, such as bovine serum albumin (BSA) and skim milk, the PEG-PLA prevents the nonspecific adsorption of several proteins. The properties of a functionalized surface were characterized by the water contact angle and atomic force microscopy (AFM). Topological analysis clearly indicated that the MIMIC method provides a reliable surface regardless of the micro- and nanopattern size. Two different functionalities of the fabricated surface produce uniform protein patterns from the micro- to nanoscale with a high signal to noise ratio. The proposed method allows for flexibility in forming shapes, such as lines, squares, circles, triangles and stars, and can control the pattern size from 400 nm to 90 μm. Finally, the antigen-antibody assay showed good linearity over the range of 10 ng/mL to 25 μg/mL, indicating its feasibility for a quantitative measurement of the concentration of target proteins in a sample.
Starting Page	868
Ending Page	875
Page Count	8
File Format	PDF
ISSN	15985032
Journal	Macromolecular Research
Volume Number	18
Issue Number	9
e-ISSN	20927673
Language	English
Publisher	The Polymer Society of Korea
Publisher Date	2010-09-23
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	poly(ethylene glycol)-poly(lactide) copolymer (PEG-PLA) polyelectrolyte multilayers (PEL) micro-molding in capillaries (MIMIC) micropatterning nanopatterning Nanotechnology Nanochemistry Characterization and Evaluation of Materials Physical Chemistry Soft and Granular Matter, Complex Fluids and Microfluidics Polymer Sciences
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Materials Chemistry Chemical Engineering Polymers and Plastics

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