NDLI: Isolation and characterization of indigenous 2,4-D herbicide degrading bacteria from an agricultural soil in proximity of Sauce Grande River, Argentina

Content Provider	Springer Nature Link
Author	Zabaloy, M. Celina Gómez, Marisa A.
Copyright Year	2013
Abstract	Our objective was to isolate and characterize indigenous bacteria able to use 2,4-D as a sole carbon (C) and energy source from an agricultural soil in the Sauce Grande River basin (Argentina). Culturable-dependant and molecular methods combined were used to identify and characterize putatively dominant indigenous degrading bacteria. Physiological traits, chloride release and biomass production showed the degradative capacity of the isolates obtained and high-performance liquid chromatography (HPLC) was used to corroborate the evidence. Degrading genes (tfdA and tfdB) were detected in all isolates, and their restriction fragment length polymorphisms (RFLP) were analyzed. Altogether, our results suggest that agricultural use of 2,4-D at recommended level leads to selection for a copiotrophic degrading population. The dominant genus able to metabolize 2,4-D in this soil was identified as Cupriavidus by 16S rRNA gene sequencing and the RFLP profiles of all isolates resembled that of Cupriavidus necator JMP134, the model organism for 2,4-D degradation. The strain EMA-G showed a remarkable performance in herbicide degradation (100 % removal in <1 day) in pure culture and is a favorite candidate for future biodegradation experiments.
Starting Page	969
Ending Page	974
Page Count	6
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	64
Issue Number	3
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-10-04
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	2,4-dichlorophenoxyacetic acid- degrading bacteria Cupriavidus tfd genes Restriction fragment length polymorphism Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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