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| Content Provider | Springer Nature Link |
|---|---|
| Author | Zhu, Duolong Zhao, Kai Xu, Haijin Zhang, Xiuming Bai, Yanling Saris, Per E. J. Qiao, Mingqiang |
| Copyright Year | 2014 |
| Abstract | Lactococcus lactis is widely used in the food industry as a food-grade non-pathogenic bacterium. In this study, we constructed the thymidylate synthase gene (thyA) deficient strain (NZ9000-3) derived from L. lactis NZ9000 using the Cre-loxP recombination system. Two loxP (lox66 and lox71) sites were integrated into the chromosome at target sites, and the thyA gene was replaced by a chloramphenicol resistance gene (cat) cassette. The recombination between the two loxP sites was very efficient when the Cre recombinase was expressed by a constructed pNZTS-Cre plasmid, and the thyA-null strain was isolated after removal of the cre gene. Then the relationship between the thyA-null strain and a thymidine requirement was analyzed. Results showed that the thyA gene was successfully knocked out, and the thyA-null strain could not grow well in minimal medium. Afterwards, pMG36e-thyA plasmid was constructed and transformed into NZ9000-3. The good screening efficiency revealed that as the selection marker thyA gene was as efficient as the erythromycin resistance gene, this suggested the thyA gene could be used as a food-grade selection marker for L. lactis to be used in food and industry applications. |
| Starting Page | 1659 |
| Ending Page | 1665 |
| Page Count | 7 |
| File Format | |
| ISSN | 15904261 |
| Journal | Annals of Microbiology |
| Volume Number | 65 |
| Issue Number | 3 |
| e-ISSN | 18692044 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-12-30 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Lactococcus lactis thyA Cre-loxP recombination system Recombinase Marker Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology |
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