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| Content Provider | Springer Nature Link |
|---|---|
| Author | Rollini, Manuela Trinetta, Valentina Musatti, Alida Manzoni, Matilde |
| Copyright Year | 2008 |
| Abstract | The aim of the present research was to investigate the influence of culture conditions on the levels of β-galactosidase (EC 3.2.1.23) activity produced byKluyveromyces strains. Interest was focused on evaluating enzyme activity levels when lactose or cheese whey was employed as substrate in culture medium formulation. From an overall look at the obtained results, the tested strains were found to be able to produce β-galactosidase at promising levels. The use of cheese whey, either for strain maintenance and production trials, allowed to obtain a high cell yield associated with β-galactosidase production. The maximum β-galactosidase volumetric activity, EA$_{ max }$ 66.5 IU/ml, corresponding to 3184 EA$_{ spec }$ IU/g cell dw, was obtained withK. marxianus MIM 782 at 37°C and 72 h incubation. |
| Starting Page | 705 |
| Ending Page | 710 |
| Page Count | 6 |
| File Format | |
| ISSN | 15904261 |
| Journal | Annals of Microbiology |
| Volume Number | 58 |
| Issue Number | 4 |
| e-ISSN | 18692044 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-01-01 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | lactase β-galactosidase cheese whey Kluyveromyces lactose hydrolysis Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Applied Microbiology and Biotechnology |
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