NDLI: Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3

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Functional characterization of two alkane hydroxylases in a versatile Pseudomonas aeruginosa strain NY3

Content Provider	Springer Nature Link
Author	Wang, Yan Nie, Maiqian Wan, Yi Tian, Xiaoting Nie, Hongyun Zi, Jing Ma, Xia
Copyright Year	2017
Abstract	Pseudomonas aeruginosa strain NY3 has an extraordinary capacity to utilize a wide range of substrates, including n–alkanes of lengths C$_{5}$ to C$_{34}$, aromatic compounds, phenols, diesel and crude oil, and it can produce a variety of small bioactive molecules, including rhamnolipids, which can enhance its metabolic capacity for hydrophobic organic pollutants. This capacity makes NY3 a good candidate for use in environmental pollution remediation. Alkane hydroxylases catalyze both the initial and rate-limiting step of the terminal oxidation of n–alkanes. To better understand the genetic mechanisms by which P. aeruginosa NY3 degrades such a wide range of n–alkanes, two putative coding genes of alkane hydroxylases were functionally characterized using a gene-knockout approach with three different degradation systems. The single n–alkane test indicated that the hydroxylase AlkB2 acted in the early growth phase and played a major role in the utilization of C$_{12}$–C$_{18}$. However, a double mutant showed a trend towards recovery when C$_{20}$–C$_{24}$ were used as sole carbon source. This suggests that there are other enzymes capable of utilizing n–alkanes longer than C$_{20}$. Tests of both artificial n–alkanes mixture and crude oil-containing waste water showed similar results, suggesting that both AlkB1 and AlkB2 are involved in n–alkane degradation, and, moreover, that AlkB2 plays a major role. Finally, given the wider functional range of both AlkBs in the mixture of n–alkanes compared to that of single n–alkanes, these results hint at co-metabolism.
Starting Page	459
Ending Page	468
Page Count	10
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	67
Issue Number	7
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-06-08
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Pseudomonas aeruginosa Alkane hydroxylase n–alkanes Crude oil-containing waste water Degradation efficiency Microbiology Microbial Genetics and Genomics Microbial Ecology Mycology Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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