NDLI: Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

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Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

Acknowledgement

Annals of Microbiology : Volume 61, Issue 3, September 2011

Annals of Microbiology : Volume 61, Issue 2, June 2011

Annals of Microbiology : Volume 61, Issue 1, March 2011

Annals of Microbiology : Volume 60

Annals of Microbiology : Volume 59

Annals of Microbiology : Volume 58

Annals of Microbiology : Volume 57

Annals of Microbiology : Volume 56

Screening for a culture medium yielding optimal colony growth, zoospore yield and infectivity of different isolates of Leptolegnia chapmanii (Straminipila: Peronosporomycetes)

Content Provider	Springer Nature Link
Author	Pelizza, Sebastian Alberto Cabello, Marta Noemi Tranchida, María Cecilia Scorsetti, Ana Clara Bisaro, Vilma
Copyright Year	2011
Abstract	Leptolegnia chapmanii is an aquatic fungus that has demonstrated marked pathogenicity towards the larvae of a number of mosquito species with little or no effect on non-target insects. The aim of this work was to determine the best culture medium for colony growth, and production and infectivity of zoospores among different isolates of L. chapmanii. No significant differences (P >0.01) were observed for the media FORT$_{(agar)}$, PYG, YPSS, and PYG$_{SF}$ in terms of colony diameter of the different isolates evaluated. In these culture media, all L. chapmanii isolates had the highest growth (45 mm$^{2}$) after 7 days. The nine isolates tested produced the greatest number of zoospores in FORT$_{(agar)}$ medium 3 (1 × 10$^{6}$ zoospores/ml). Medium YPSS exhibited significant differences (P <0.0001) compared to other culture media with respect to maintenance of virulence and infectivity of the isolates, producing 100% larval mortality after 48 h of inoculation. Thus, for the cultivation and maintenance of L. chapmanii isolates, the medium of choice among those tested is FORT$_{(agar)}$, as it is both inexpensive to prepare and ensures optimal colony development, extremely high zoospore production, and excellent maintenance of fungal infectivity.
Starting Page	991
Ending Page	997
Page Count	7
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	61
Issue Number	4
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-02-24
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Leptolegnia chapmanii Isolate Culture medium Aedes aegypti Fungus Genetics Applied Microbiology Microbiology Microbial Genetics and Genomics Microbial Ecology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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Production of Oogonia and Oospores of Leptolegnia chapmanii Seymour (Straminipila: Peronosporomycetes) in Aedes aegypti (L.) Larvae at Different Temperatures

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