NDLI: Pseudomonas fluorescens alter whole-voltage-activated currents of cultured rat cerebellar granule neurons

Pseudomonas fluorescens alter whole-voltage-activated currents of cultured rat cerebellar granule neurons

Content Provider Springer Nature Link
Author Mezghani, Sana Lesouhaitier, Olivier Merieau, Annabelle Chevalier, Sylvie Orange, Nicole Feuilloley, Marc Cazin, Lionel
Copyright Year 2009
Abstract In the present study the effect of infection on the resting membrane potential and whole-voltage-activated currents of neurons was determined usingPseudomonas fluorescens and lipopolysaccharide (LPS) extracted from the same species. Electrophysiological studies were performed on cerebellar granule neurons using the whole cell configuration of the patch clamp technique. The resting potential of cells (−63.7±2 mV) was significantly less negative (−46.0±4.7 mV) in cells showing adherent bacteria (10$^{6}$ CFU/ml). In addition, the whole-voltage currents triggered by voltage pulses were markedly reduced in neurons incubated withP. Fluorescens (mean 58%). Treatment with LPS (200 ng/ml) extracted fromP. Fluorescens provoke also a significant depolarisation of the membrane of the granule neurons and a severely alteration of whole-voltage currents. Analysis of the current-voltage relationships of the peak currents revealed the implication of potassium channels in the response of the cells toP. Fluorescens and its LPS. The present report provides the first data on the effect of a living bacterium on the membrane electrical activity of neurons and comforts the recent observations of the high cytotoxic potential ofP. fluorescens.
Starting Page 379
Ending Page 382
Page Count 4
File Format PDF
ISSN 15904261
Journal Annals of Microbiology
Volume Number 59
Issue Number 2
e-ISSN 18692044
Language English
Publisher Springer-Verlag
Publisher Date 2009-01-01
Publisher Place Berlin, Heidelberg
Access Restriction Subscribed
Subject Keyword patch-clamp cerebellar granule neurons lipopolysccharide Pseudomonas fluorescens voltage-activated currents Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type Text
Resource Type Article
Subject Applied Microbiology and Biotechnology

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Development of Voltage-Activated Potassium Currents in Cultured Cerebellar Granule Neurons Under Different Growth Conditions

Pseudomonas fluorescens alter whole-voltage-activated currents of cultured rat cerebellar granule neurons

Content Provider	Springer Nature Link
Author	Mezghani, Sana Lesouhaitier, Olivier Merieau, Annabelle Chevalier, Sylvie Orange, Nicole Feuilloley, Marc Cazin, Lionel
Copyright Year	2009
Abstract	In the present study the effect of infection on the resting membrane potential and whole-voltage-activated currents of neurons was determined usingPseudomonas fluorescens and lipopolysaccharide (LPS) extracted from the same species. Electrophysiological studies were performed on cerebellar granule neurons using the whole cell configuration of the patch clamp technique. The resting potential of cells (−63.7±2 mV) was significantly less negative (−46.0±4.7 mV) in cells showing adherent bacteria (10$^{6}$ CFU/ml). In addition, the whole-voltage currents triggered by voltage pulses were markedly reduced in neurons incubated withP. Fluorescens (mean 58%). Treatment with LPS (200 ng/ml) extracted fromP. Fluorescens provoke also a significant depolarisation of the membrane of the granule neurons and a severely alteration of whole-voltage currents. Analysis of the current-voltage relationships of the peak currents revealed the implication of potassium channels in the response of the cells toP. Fluorescens and its LPS. The present report provides the first data on the effect of a living bacterium on the membrane electrical activity of neurons and comforts the recent observations of the high cytotoxic potential ofP. fluorescens.
Starting Page	379
Ending Page	382
Page Count	4
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	59
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-01-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	patch-clamp cerebellar granule neurons lipopolysccharide Pseudomonas fluorescens voltage-activated currents Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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