NDLI: Use of chitinolytic Bacillus atrophaeus strain S2BC-2 antagonistic to Fusarium spp. for control of rhizome rot of ginger

Content Provider	Springer Nature Link
Author	Shanmugam, Veerubommu Thakur, Himankshi Gupta, Samriti
Copyright Year	2012
Abstract	An antagonistic bacillus S2BC-2 isolated from apple rhizosphere soil was identified to be highly chitinolytic on chitinase detection agar. Standard bacteriological tests and sequencing of 16S rRNA, and gyrA and rpoB genes, indicated a taxonomic affiliation of the strain to Bacillus atrophaeus. The strain was studied for its ability to grow and produce chitinase on different substrates. Bacterial cells grown on chitin-containing media showed enhanced growth and chitinase production with increased anti-fungal activity against vascular wilt pathogens. Extracellular proteins of cell-free extracts of media amended with chitin and fungal cell wall contained 4–10 novel polypeptides. In polyhouse (bamboo structures that provide protective shade made of polyvinyl sheet) studies, a chitin-supplemented talc-based formulation of the S2BC-2 challenge inoculated with Fusarium oxysporum f. sp. zingiberi recorded low percent disease indices of 84.9 % and 79.2 % for yellows and rhizome rot, respectively, over the non-bacterised pathogen control. The low disease incidences correlated with 113.3 % maximum rhizome production and 2-fold higher chitinase induction over the pathogen control. In native gel activity assays, upon challenge-inoculation, S2BC-2 expressed more chitinase isoforms than the pathogen control. The results suggest that chitinolytic B. atrophaeus can be used in the biocontrol of rhizome rot of ginger.
Starting Page	989
Ending Page	996
Page Count	8
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	63
Issue Number	3
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2012-10-05
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Fusarium oxysporum Ginger Bacillus atrophaeus Chitinolysis Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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