NDLI: Isolation and characterization of biofilm-forming bacteria and associated extracellular polymeric substances from oral cavity

Content Provider	Springer Nature Link
Author	Jain, Kirti Parida, Sheetal Mangwani, Neelam Dash, Hirak R. Das, Surajit
Copyright Year	2013
Abstract	The current work deals with the studies on characterization of two biofilm-forming bacteria isolated from the oral cavity. The major constituent of biofilm other than bacterial cells is the extracellular polymeric substance (EPS) matrix, which is secreted by the bacterial cells themselves. Physical properties of biofilms such as attachment, mechanical strength, antibiotic resistance can be attributed to EPS matrix. Molecular phylogeny confirmed these two isolates as Pseudomonas aeruginosa and Bacillus subtilis. It was observed that cell attachment in both the strains was maximal when xylose was used as the sole carbon source. The EPS characterization result indicated the presence of a macromolecular complex constituting of carbohydrate, protein, lipids and nucleic acids. Test for biofilm formation in the presence of metal salts of iron and zinc showed moderate to high inhibition of biofilm formation. However, calcium, iron and copper have been found to enhance biofilm growth significantly. There was more than 50 % increase in biofilm growth by P. aeruginosa with an increase in calcium concentration up to 80 ppm (Two tailed t-test P < 0.05), whereas ≥ 15 % increase in biofilm growth by B. subtilis was observed in the presence of 80 ppm of calcium. However, variations were significant (Two way ANOVA, P < 0.01) between different metals in different concentrations. In this study, attempts have been made to examine the effect of different carbon sources and physiological conditions on biofilm growth.
Starting Page	1553
Ending Page	1562
Page Count	10
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	63
Issue Number	4
e-ISSN	18692044
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-02-19
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Biofilms EPS Xylose Macromolecules Pseudomonas Bacillus Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biofilms: Extracellular Bastions of Bacteria

A novel integrated approach to quantitatively evaluate the efficiency of extracellular polymeric substances (EPS) extraction process

Extraction of extracellular polymeric substances from extreme acidic microbial biofilms

Study of extracellular polymeric substances in the biofilms of a suspended biofilter for nitric oxide removal

Effects of short-time drying on biofilm-associated bacteria

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

Soluble microbial products (SMP) and soluble extracellular polymeric substances (EPS) from wastewater sludge

Formation of extracellular polymeric substances from acidogenic sludge in H2-producing process

Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans

Isolation and characterization of biofilm-forming bacteria and associated extracellular polymeric substances from oral cavity

Similar Documents

Biofilms: Extracellular Bastions of Bacteria

A novel integrated approach to quantitatively evaluate the efficiency of extracellular polymeric substances (EPS) extraction process

Extraction of extracellular polymeric substances from extreme acidic microbial biofilms

Study of extracellular polymeric substances in the biofilms of a suspended biofilter for nitric oxide removal

Effects of short-time drying on biofilm-associated bacteria

Extraction of extracellular polymeric substances (EPS) from anaerobic granular sludges: comparison of chemical and physical extraction protocols

Soluble microbial products (SMP) and soluble extracellular polymeric substances (EPS) from wastewater sludge

Formation of extracellular polymeric substances from acidogenic sludge in H2-producing process

Characterization of extracellular polymeric substances from denitrifying organism Comamonas denitrificans

Isolation and characterization of biofilm-forming bacteria and associated extracellular polymeric substances from oral cavity