NDLI: Production of tyrosinase by Auricularia auricula using low cost fermentation medium

Content Provider	Springer Nature Link
Author	Zou, Yu Hu, Wenzhong Liu, Chenghui Chen, Chen Gu, Zhenxin
Copyright Year	2012
Abstract	Low cost fermentation media using agricultural by-products (wheat bran extract, rice bran extract and soybean meal extract) as a major nutrient source, were evaluated for the production of tyrosinase from the fungus Auricularia auricula in submerged culture. In single-factor experiments, three components (wheat bran extract, casein and CuSO$_{4}$) were chosen to further optimize medium composition using response surface methodology (RSM). The central composite experimental results showed the following optimum medium composition: wheat bran extract 36.0 %, casein 1.1 g/l and CuSO$_{4}$ 0.13 g/l. Under these conditions, the highest tyrosinase activity was 17.22 U/ml, which was 2.1 fold higher than that obtained using the non-optimized medium. The present study is the first to report the statistical optimization of medium composition for production of tyrosinase by A. auricula using cheaper wheat bran extract as a major nutrient source. These results might provide a reference for the development of a cost-effective medium for commercial production of tyrosinase.
Starting Page	699
Ending Page	705
Page Count	7
File Format	PDF
ISSN	15904261
Journal	Annals of Microbiology
Volume Number	63
Issue Number	2
e-ISSN	18692044
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-09-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Auricularia auricula Tyrosinase Wheat bran extract RSM optimization Submerged fermentation Microbiology Microbial Genetics and Genomics Microbial Ecology Fungus Genetics Medical Microbiology Applied Microbiology
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology

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