NDLI: Continuous oxygen supply in pump-less micro-bioreactor based on microfluidics

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Continuous oxygen supply in pump-less micro-bioreactor based on microfluidics

Content Provider	Springer Nature Link
Author	Marimuthu, Mohana Kim, Sanghyo
Copyright Year	2014
Abstract	This research aims a continuous and uniform oxygen tensions and oxygen gradients supply in microfluidic cell culture chip based micro-bioreactor without any external pumps by modifying the existing siphon based perfusion strategy using conventional tools to control constant hydrostatic pressure for constant fluid flow rate. In this study, the microfluidic based micro-bioreactor is fabricated using a polydimethylsiloxane (PDMS) replication process. The micro-bioreactor chip is composed of simple oxygen perfusion setup connecting to capillary-like branched microchannels and a cell culture and support loading area. The function of the new pump-less fluid perfusion in microfluidic cell culture chip and maintains constant oxygen supply in the cell culture area has been experimentally evaluated. And the results demonstrate that this proposed microfluidic flow system in a micro-bioreactor is proved to provide a linear distribution of oxygen throughout the cell culture area without any external and internal interference. Also, oxygen diffusion into the culture area through a microscale channel has been successfully established by the constant hydraulic pressure controlled by siphoning effect. Such flow system was used in a PDMS based microfluidic micro-bioreactor design to provide the high-throughput oxygen diffusion and perfusion, and the uniform oxygen/nutrient distribution continuously supplied for dermal fibroblast cell culture. This could be a potential and effective model to be incorporated into tissue regeneration studies, drug screening model, and in cancer tissue model studies for understanding angiogenesis, where oxygen tension and perfusion cultures play important roles.
Starting Page	1
Ending Page	9
Page Count	9
File Format	PDF
ISSN	19760280
Journal	BioChip Journal
Volume Number	9
Issue Number	1
e-ISSN	20927843
Language	English
Publisher	The Korean BioChip Society (KBCS)
Publisher Date	2014-12-29
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Micro-bioreactor Dermal fibroblast cell Pump-less perfusion Oxygen diffusion Hydrostatic pressure Biotechnology Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Electrical and Electronic Engineering Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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