NDLI: A multi-inlet microfluidic device fabricated for in situ detection of multiple cytotoxicity endpoints

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BioChip Journal : Volume 5

BioChip Journal : Volume 4

A multi-inlet microfluidic device fabricated for in situ detection of multiple cytotoxicity endpoints

Content Provider	Springer Nature Link
Author	Mahto, Sanjeev Kumar Rhee, Seog Woo
Copyright Year	2012
Abstract	This work describes the development of a multi-inlet microfluidic device for in situ detection of multiple cytotoxicity endpoints. The device consists of an upstream microfluidic multi-inlet module (μMIM) and a downstream microfluidic cell culture channel (μCCC). The integration of a device with syringe pumps via the inlets of MIM mainly enables the performance of multiplex cytotoxicity assays targeted on multiple endpoints. The device in the study was used for the long-term culturing of BALB/3T3 fibroblast cells. In addition, cadmium ion was used as a toxicant to induce the toxicity in the cultured cells. The cytotoxicity endpoints, such as the in situ detection of reactive oxygen species (ROS), nuclear structures and cell morphology, were examined and correlated simultaneously for every single cell after they were exposed to cadmium. The data indicated that the percentage of cells that had produced ROS and shown nuclear staining were slightly higher than the cells that had produced ROS only, but this percentage was significantly higher than the cells that had shown only nuclear staining. The results revealed that the obtained correlated data from simultaneous observations of multiplex cytotoxicity assays were more efficient in providing the mechanistic explanation for Cd-induced toxicity than the individual data that were derived after examining a single endpoint. In addition, the results suggested that the intracellular formation of ROS induced by cadmium might have been followed by the alterations in cell and nuclear morphology and loss of membrane integrity. The device allows for the real-time monitoring of cells and their behavior against a toxicant and facilitates the rapid performance of cytotoxicity testing. Furthermore, we anticipate that the presented device could be very effective in advancing the understanding of the precise mechanism of cytotoxicity by improving the sensitivity and accuracy in identifying endpoints.
Starting Page	48
Ending Page	55
Page Count	8
File Format	PDF
ISSN	19760280
Journal	BioChip Journal
Volume Number	6
Issue Number	1
e-ISSN	20927843
Language	English
Publisher	The Korean BioChip Society (KBCS)
Publisher Date	2012-03-20
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Microfluidics Multi-inlet module Cytotoxicity endpoints Multiplex Cytotoxicity assays Biotechnology Biomedical Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Electrical and Electronic Engineering Biotechnology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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