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| Content Provider | Springer Nature Link |
|---|---|
| Author | Armaleo, Daniele May, Susan |
| Copyright Year | 2009 |
| Abstract | Using a method based on quantitative PCR, we determined that the nuclear genome sizes for the mycobiont and photobiont of the lichen Cladonia grayi are 28.6 Mb and 106.7 Mb, respectively. This is the first genome size determination for lichens, and suggests that between 20,000 and 25,000 genes function in C. grayi. The mycobiont genome size is near the middle of the range observed within the Pezizomycota, the subphylum containing all known ascomycete lichen fungi. The genome size of the photobiont (the green alga Asterochloris sp.) is near the lower end of its class, the Trebouxiophyceae. Genomes in this size range can be sequenced at relatively low cost with current pyrosequencing-based methods. The genome sizing method requires very small amounts of precisely quantified DNA and should be applicable to any lichen whose symbionts can be reliably isolated from one another. Since the symbionts used in this project were isolated from soredia, the lichen’s vegetative propagules, we also describe a method for the establishment of axenic symbiont cultures from large numbers of soredia. |
| Starting Page | 43 |
| Ending Page | 51 |
| Page Count | 9 |
| File Format | |
| ISSN | 03345114 |
| Journal | Symbiosis |
| Volume Number | 49 |
| Issue Number | 1 |
| e-ISSN | 18787665 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-10-31 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Genome size qPCR Cladonia lichens lichenoids soredia culture symbiosis mycobiont photobiont Asterochloris Ecology Plant Sciences Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Agricultural and Biological Sciences |
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