NDLI: Inter- and intrasporal nuclear ribosomal gene sequence variation within one isolate of arbuscular mycorrhizal fungus, Diversispora sp.

Content Provider	Springer Nature Link
Author	Thiéry, Odile Moora, Mari Vasar, Martti Zobel, Martin Öpik, Maarja
Copyright Year	2012
Abstract	Most molecular ecological studies of arbuscular mycorrhizal fungi (AMF) have been based on the rRNA gene sequences. However, information about intraspecific nucleotide variation is still limited in these fungi. In this study, we calculated the inter- and intrasporal nucleotide variation of Diversispora sp. EE1 using 78 cloned sequences from four spores within a ca 4960 bp fragment of the nuclear ribosomal operon spanning the near full length small ribosomal subunit (SSU) rRNA gene, the full internal transcribed spacer (ITS: ITS1-5.8S-ITS2) and ca 2740 bp of the large ribosomal subunit (LSU) rRNA gene. Data for each marker region (SSU, ITS and LSU) originated from the very same spores. Sequence variation resulting from point mutations and small indels was recorded in all regions. Highest sequence variation was observed in the ITS region at both the inter- and intrasporal levels. The ITS1 component was more variable than ITS2, whilst the 5.8S gene was the least variable component of the ITS region. Evolutionary divergence of gene copies between spores was intermediate for the LSU and lowest for the SSU. The SSU and the LSU genes had relatively similar evolutionary divergence per spore. Sequence variant richness was not exhaustive for any of the marker regions, indicating that multiple sequences per spore from multiple spores are needed when characterizing a species. This study provides reference sequences for ecological studies, permitting identification of AMF using any of the ribosomal regions or primer systems.
Starting Page	135
Ending Page	147
Page Count	13
File Format	PDF
ISSN	03345114
Journal	Symbiosis
Volume Number	58
Issue Number	1-3
e-ISSN	18787665
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-12-29
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Arbuscular mycorrhizal fungi Diversispora Intersporal Intrasporal rDNA variation Evolutionary Biology Developmental Biology Microbiology Plant Sciences Ecology
Content Type	Text
Resource Type	Article
Subject	Agricultural and Biological Sciences

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