NDLI: Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool

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Measurement of N$_{2}$ fixation in 30 cowpea (Vigna unguiculata L. Walp.) genotypes under field conditions in Ghana, using the$^{15}$N natural abundance technique

Effect of legume plant density and mixed culture on symbiotic N$_{2}$ fixation in five cowpea (Vigna unguiculata L. Walp.) genotypes in South Africa

Assessing the symbiotic dependency of grain and tree legumes on N$_{2}$ fixation for their N nutrition in five agro-ecological zones of Botswana

Indigenous legumes biomass quality and influence on C and N mineralization under indigenous legume fallow systems

Germination, field establishment patterns and nitrogen fixation of indigenous legumes on nutrient-depleted soils

Nitrogen and carbon costs of soybean and lupin root systems during phosphate starvation

Sequence analysis of a DNA fragment fromSinorhizobium fredii USDA257 which extends the nitrogen fixation host range ofRhizobium species NGR234 to soybean,Glycine max (L.) Merr cultivar Peking

Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool

Genetic diversity ofBradyrhizobium japonicum within soybean growing regions of the north-eastern Great Plains of North America as determined by REP-PCR and ERIC-PCR profiling

Symbiotic performance of selectedCyclopia Vent. (honeybush) rhizobia under nursery and field conditions

Nitrogen fixation with the soybean crop in Brazil: Compatibility between seed treatment with fungicides and bradyrhizobial inoculants

Characterization of fungal soil communities by F-RISA and arbuscular mycorrhizal fungi fromAraucaria angustifolia forest soils after replanting and wildfire disturbances

Thin-layer chromatographic analysis of lumichrome, riboflavin and indole acetic acid in cell-free culture filtrate ofPsoralea nodule bacteria grown at different pH, salinity and temperature regimes

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Symbiosis : Volume 47

Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool

Content Provider	Springer Nature Link
Author	Menna, Pâmela Pereira, Alan Alves Bangel, Eliane Villamil Hungria, Mariangela
Copyright Year	2009
Abstract	With more than 30 million doses of rhizobial inoculants marketed per year, it is probable that Brazilian agriculture benefits more than any other country from symbiotic N$_{2}$ fixation. As a result of strain-selection programs, 142 strains of rhizobia are officially recommended for use in commercial inoculants for ninety-six leguminous crops. In this study, sixty-eight of these elite strains were characterized by rep-PCR with the BOX-primer. Reproducibility of the DNA profiles was confirmed, suggesting efficacy of BOX-PCR both for control of quality of inoculants and for preliminary characterization of rhizobial culture collections. Strains of different species never showed similarity higher than 70% in the BOX-PCR analysis, however, some strains of the same species fit into more than one cluster, and correlation between BOX-PCR products and l6S rRNA sequences was low (7.6%). On the other hand, a polyphasic approach — 20%∶80% of BOX-PCR:16S rRNA which correlated well with the l6S rRNA analysis (95%), and provided higher definition of the genotypes, resulting in clearer indications of the taxonomic groups — might expedite rhizobial diversity studies.
Starting Page	120
Ending Page	130
Page Count	11
File Format	PDF
ISSN	03345114
Journal	Symbiosis
Volume Number	48
Issue Number	1-3
e-ISSN	18787665
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacterial fingerprinting bacterial taxonomy biological nitrogen fixation Rhizobiales inoculants 16S rRNA Microbiology Plant Sciences Ecology
Content Type	Text
Resource Type	Article
Subject	Agricultural and Biological Sciences

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Genetic diversity of elite rhizobial strains of subtropical and tropical legumes based on the 16S rRNA and glnII genes

Editorial: Maximizing Nitrogen Fixation in Legumes as a Tool for Sustainable Agriculture Intensification

Genetic Diversity of Rhizobia Nodulating Arachis hypogaea L. in Central Argentinean Soils

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Diversity and symbiotic effectiveness of beta-rhizobia isolated from sub-tropical legumes of a Brazilian Araucaria Forest

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Rep-PCR of tropical rhizobia for strain fingerprinting, biodiversity appraisal and as a taxonomic and phylogenetic tool

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