NDLI: Development of menaquinone-7 enriched nutraceutical: inside into medium engineering and process modeling

Content Provider	Springer Nature Link
Author	Singh, Rishipal Puri, Alka Panda, Bibhu Prasad
Copyright Year	2014
Abstract	Menaquinone 7 (MK-7) is nutritionally important metabolite found by fermentation mainly using B. subtilis species. In this study, soybean medium was modified to improve the MK-7 production using Bacillus subtilis NCIM 2708 under solid state fermentation. The objective of this study was to produce large amount of MK-7 within a short period of time. Nine nutritional components viz. glycerol, mannitol, dextrose, sucrose, yeast extract, malt extract, K$_{2}$HPO$_{4}$, MgSO$_{4}$.7H$_{2}$O and CaCl$_{2}$ were investigated to obtain the maximum MK-7 concentration. The highest MK-7 concentration 39.039 μg/g was obtained after 24 h of fermentation in the following optimised medium components: soybean 20 g, glycerol 40 ml/kg, mannitol 60 g/kg, yeast extract 4 g/kg, malt extract 8 g/kg and calcium chloride 4 g/kg. The maximum production of MK-7 56.757 μg/g was predicted by point prediction tool of Design Expert 7.1 software (Statease Inc. USA). This data shows 68.78 % validity of the predicted model.
Starting Page	5212
Ending Page	5219
Page Count	8
File Format	PDF
ISSN	00221155
Journal	Journal of Food Science and Technology
Volume Number	52
Issue Number	8
e-ISSN	09758402
Language	English
Publisher	Springer India
Publisher Date	2014-10-19
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Soybean Solid state fermentation Menaquinone-7 Medium engineering Food Science Nutrition Chemistry/Food Science
Content Type	Text
Resource Type	Article
Subject	Food Science

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