NDLI: Evaluation of reference genes for quantitative real-time PCR analysis of gene expression during early development processes of the tongue sole (Cynoglossus semilaevis)

Content Provider	Springer Nature Link
Author	Ma, Qian Zhuang, Zhimeng Feng, Wenrong Liu, Shufang Tang, Qisheng
Copyright Year	2015
Abstract	Differential expression of genes is crucial to growth and development of fish. To select the appropriate genes for gene normalization during Cynoglossus semilaevis early developmental process, eight candidate reference genes (ACTB, B2M, EF1A, GADPH, RPL7, TUBA, UBCE and 18S) were tested for their adequacy by using quantitative real-time PCR. The results showed that the expression of all the examined genes exhibited tissue dependent variations in the mature C. semilaevis. EF1A was listed as the most stable reference among the 14 tissues by RefFinder. Furthermore, the recommended comprehensive ranking of the stability determined by RefFinder showed that 18S was the most stable gene during the early developmental stages (from oosphere to 90 days old) in this study. However, when divided the C $_{t}$ value data of the above mentioned early developmental stages into two separate periods (embryo and post-hatching periods), TUBA and 18S represented the most stable references of these two developmental periods, respectively. Consequently, the reference gene should be carefully and accurately chosen even for studies of the same species at various developmental processes. The relevant data may help in selecting appropriate reference genes for mRNA expression analysis, and is of great value in the studies of fish growth and development.
Starting Page	90
Ending Page	97
Page Count	8
File Format	PDF
ISSN	0253505X
Journal	Acta Oceanologica Sinica
Volume Number	34
Issue Number	10
e-ISSN	18691099
Language	English
Publisher	The Chinese Society of Oceanography
Publisher Date	2015-08-26
Publisher Place	Beijing
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	quantitative real-time PCR reference gene early development Cynoglossus semilaevis Oceanography Climatology Ecology Engineering Fluid Dynamics Marine & Freshwater Sciences Environmental Chemistry
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Oceanography

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