NDLI: Tumor necrosis factor-α-induced microvascular endothelial cell hyperpermeability: role of intrinsic apoptotic signaling

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Tumor necrosis factor-α-induced microvascular endothelial cell hyperpermeability: role of intrinsic apoptotic signaling

Content Provider	Springer Nature Link
Author	Sawant, Devendra A. Wilson, Rickesha L. Tharakan, Binu Stagg, Hayden W. Hunter, Felicia A. Childs, Ed W.
Copyright Year	2014
Abstract	Tumor necrosis factor-α (TNF-α), a pro-apoptotic cytokine, is involved in vascular hyperpermeability, tissue edema, and inflammation. We hypothesized that TNF-α induces microvascular hyperpermeability through the mitochondria-mediated intrinsic apoptotic signaling pathway. Rat lung microvascular endothelial cells grown on Transwell inserts, chamber slides, or dishes were treated with recombinant TNF-α (10 ng/ml) in the presence or absence of a caspase-3 inhibitor, Z-DEVD-FMK (100 μM). Fluorescein isothiocyanate (FITC)-albumin (5 mg/ml) was used as a marker of monolayer permeability. Mitochondrial reactive oxygen species (ROS) was determined using dihydrorhodamine 123 and mitochondrial transmembrane potential using JC-1. The adherens junction integrity and actin cytoskeletal organization were studied using β-catenin immunofluorescence and rhodamine phalloidin, respectively. Caspase-3 activity was measured fluorometrically. The pretreatment with Z-DEVD-FMK (100 μM) attenuated TNF-α-induced (10 ng/ml) disruption of the adherens junctions, actin stress fiber formation, increased caspase-3 activity, and monolayer hyperpermeability (p < 0.05). TNF-α (10 ng/ml) treatment resulted in increased mitochondrial ROS formation and decreased mitochondrial transmembrane potential. Intrinsic apoptotic signaling-mediated caspase-3 activation plays an important role in regulating TNF-α-induced endothelial cell hyperpermeability.
Starting Page	971
Ending Page	980
Page Count	10
File Format	PDF
ISSN	11387548
Journal	Journal of Physiology and Biochemistry
Volume Number	70
Issue Number	4
e-ISSN	18778755
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-11-14
Publisher Institution	University of Navarra, Spain
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Vascular permeability Adherens junction VE-cadherin β-Catenin Mitochondria Caspase-3 Biomedicine general Human Physiology Animal Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Biochemistry

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