NDLI: Assessing the Quality and Reproducibility of a Proteomic Platform for Clinical Stroke Biomarker Discovery

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Proteomic Protease Substrate Profiling of tPA Treatment in Acute Ischemic Stroke Patients: A Step Toward Individualizing Thrombolytic Therapy at the Bedside

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Assessing the Quality and Reproducibility of a Proteomic Platform for Clinical Stroke Biomarker Discovery

Translational Stroke Research : Volume 1, Issue 3, September 2010

Translational Stroke Research : Volume 1, Issue 2, June 2010

Translational Stroke Research : Volume 1, Issue 1, March 2010

Assessing the Quality and Reproducibility of a Proteomic Platform for Clinical Stroke Biomarker Discovery

Content Provider	Springer Nature Link
Author	Sideso, Ediri Papadakis, Michalis Wright, Cynthia Handa, Ashok Buchan, Alastair Kessler, Benedikt Kennedy, James
Copyright Year	2010
Abstract	The aim of this study was to investigate the quality and reproducibility of mass spectra derived from a matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) platform in a patient population undergoing carotid endarterectomy. Plasma samples were either digested with trypsin or left undigested, fractionated with either C18 or weak cation exchange (WCX) columns and analysed by MALDI-TOF MS. Quality of mass spectra for each method was assessed by baseline correction (lower area under the curve ratio indicating higher quality) and signal-to-noise ratio. Mean coefficient of variation (CV%) assessed reproducibility between repeated experiments and methods. Identified mass peak intensity differences were assessed for consistency across repeated experiments. Plasma from six patients was analysed. The quality of mass spectra was significantly better when derived from digested plasma fractionated by either WCX or C18 methods compared to undigested plasma fractionated by WCX (analysis of variance, p < 0.05). Mean CV% for repeated experiments was 18% and 28% for WCX and C18 fractionated digested plasma, respectively. A small number of differences in mass peak intensities were consistently observed in repeated experiments. Repeated experiments are required to confidently identify non-random mass peak intensity differences as putative plasma biomarkers that merit further investigation.
Starting Page	304
Ending Page	314
Page Count	11
File Format	PDF
ISSN	18684483
Journal	Translational Stroke Research
Volume Number	1
Issue Number	4
e-ISSN	1868601X
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-14
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cerebral Ischaemia Proteomics Biomarker discovery MALDI-TOF C18 Weak cation exchange Vascular Surgery Neurosurgery Cardiology Neurology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cardiology and Cardiovascular Medicine Neuroscience Neurology (clinical)

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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