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Enhanced Procoagulant Activity on Blood Cells after Acute Ischemic Stroke

Content Provider	Springer Nature Link
Author	Yao, Zhipeng Wang, Lixiu Wu, Xiaoming Zhao, Lu Chi, Chunling Guo, Li Tong, Dongxia Yang, Xiaoyan Dong, Zengxiang Deng, Ruijuan Novakovic, Valerie A. Thatte, Hemant S Bi, Yayan Tian, Ye Shi, Jialan Zhou, Jin Kou, Junjie Hu, Shaoshan
Copyright Year	2016
Abstract	The role of phosphatidylserine (PS)-mediated procoagulant activity (PCA) in stroke remains unclear. To ascertain this role, early dynamic evolution of PS exposure on blood cells and released microparticles (MPs) and the corresponding PCA were evaluated in patients with acute ischemic stroke (AIS). Flow cytometry analyses revealed that initial levels of PS exposure on erythrocyte, platelet, and leukocyte were 2.40-, 1.36-, and 1.38-fold higher, respectively, in AIS than the risk factor-matched (RF) controls. Concomitantly, total PS$^{+}$ MPs were increased in AIS (1949 ± 483/μl) compared with the RF group (1674 ± 387/μl; P = 0.019) and healthy controls (1052 ± 179/μl; P < 0.001). Specifically, PS$^{+}$ erythrocytes gradually increased within 1 week. PS$^{+}$ platelets and MPs peaked at 24 h and declined at 7 days, while PS$^{+}$ leukocytes were markedly elevated at 24 h. Further, PS exposure on blood cells and MPs in stroke resulted in shortened clotting time with an accompanying increase in FXa and thrombin formation significantly. Treatment with lactadherin, a PS antagonist, delayed the coagulation time by approximately 20 % and blocked the generation of FXa and thrombin by about 50 %. Furthermore, initial counts of PS$^{+}$ platelets and platelet MPs significantly correlated with stroke severity. Thrombin generation promoted by platelets and MPs at 12 h was significantly higher in patients with cardioembolism than in patients without. The thrombophilic susceptibility of AIS patients can be partly ascribed to PS exposure on blood cells and the release of MPs. Our studies identify PS exposure as a potentially novel therapeutic target in the treatment of AIS.
Starting Page	83
Ending Page	91
Page Count	9
File Format	PDF
ISSN	18684483
Journal	Translational Stroke Research
Volume Number	8
Issue Number	1
e-ISSN	1868601X
Language	English
Publisher	Springer US
Publisher Date	2016-09-20
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Acute ischemic stroke Procoagulant Phosphatidylserine Microparticles Thrombin Neurosciences Neurology Cardiology Neurosurgery Vascular Surgery
Content Type	Text
Resource Type	Article
Subject	Cardiology and Cardiovascular Medicine Neuroscience Neurology (clinical)

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