NDLI: Trichomes and regeneration by direct organogenesis of medicinal plant Dracocephalum kotschyi L. using shoot tips (Lamiaceae)

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Trichomes and regeneration by direct organogenesis of medicinal plant Dracocephalum kotschyi L. using shoot tips (Lamiaceae)

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Trichomes and regeneration by direct organogenesis of medicinal plant Dracocephalum kotschyi L. using shoot tips (Lamiaceae)

Content Provider	Springer Nature Link
Author	Kosar, Moradi Mahmoud, Otroshy
Copyright Year	2012
Abstract	The present study describes the procedure for micropropagation of Dracocephalum kotschyi L. using shoot tips from in vitro-germinated plants. The best response was observed for shoot tips on MS medium containing 5 mg 6-benzylaminopurine L$^{−1}$ and 0.2 mg 1-naphthaleneacetic L$^{−1}$ acid. Regeneration for other types of the explant hypocotyls and cotyledons did not show satisfactory results so that the explants did not develop into normal shoots and in turn developed into the calli after 12 days of culture. Histochemical analysis showed that only the shoot tip revealed a direct induction of more teratological protuberances that arise around the cut end of the explants. Elongation of shoot buds was obtained on MS medium containing 1 mg BAP L$^{−1}$ + 0.5 mg IBA L$^{−1}$. Regenerated shoots rooted best on the same medium of elongation. After hardening, the rooted plants were transferred to the greenhouse where they grew, matured, and flowered normally with a survival rate of 95%. We concluded that the present protocol can be efficiently used for mass propagation of Dracocephalum kotschyi.
Starting Page	251
Ending Page	257
Page Count	7
File Format	PDF
ISSN	19759479
Journal	Journal of Crop Science and Biotechnology
Volume Number	15
Issue Number	3
e-ISSN	20058276
Language	English
Publisher	The Korean Society of Crop Science
Publisher Date	2012-09-29
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Dracocephalum kotschyi organogenesis regeneration trichomes Plant Sciences Plant Physiology Biotechnology Plant Pathology Plant Genetics & Genomics
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology Agronomy and Crop Science

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