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| Content Provider | Springer Nature Link |
|---|---|
| Author | Kumar, Arthikala Manoj Sreevathsa, Rohini Reddy, Kalpana Nanja Ganesh, Prasa Trichy Udayakumar, Makarla |
| Copyright Year | 2011 |
| Abstract | Agrobacterium tumefaciens mediated in planta transformation protocol was developed for castor, Ricinus communis. Two-day-old seedlings were infected with Agrobacterium strain EHA105/pBinBt8 harboring cry1AcF and established in the greenhouse. Screening the T$_{1}$ generation seedlings on 300 mg L$^{−1}$ kanamycin identified the putative transformants. Molecular and expression analysis confirmed the transgenic nature and identified high-expressing plants. Western blot analysis confirmed the co-integration of the nptII gene in the selected transgenic plants. Bioassay against Spodoptera litura corroborated with high expression and identified five promising effective lines. Analysis of the T$_{2}$ generation plants proved the stability of the transgene indicating the feasibility of the method. |
| Starting Page | 125 |
| Ending Page | 132 |
| Page Count | 8 |
| File Format | |
| ISSN | 19759479 |
| Journal | Journal of Crop Science and Biotechnology |
| Volume Number | 14 |
| Issue Number | 2 |
| e-ISSN | 20058276 |
| Language | English |
| Publisher | The Korean Society of Crop Science |
| Publisher Date | 2011-07-06 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | cry1AcF gene in planta transformation insect resistance Ricinus communis Spodoptera litura Plant Pathology Plant Genetics & Genomics Plant Sciences Plant Physiology Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Biotechnology Agronomy and Crop Science |
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