NDLI: Isolation of eleven polymorphic microsatellite loci for the endangered Sillago parvisquamis and cross-species amplification with Sillago japonica

Content Provider	Springer Nature Link
Author	Umino, Tetsuya Ueno, Kanako Mihara, Takeshi Koike, Masaru Watanabe, Makoto Ahmad Syazni, Kamarudin Ishitani, Makoto Ohara, Kenichi
Copyright Year	2013
Abstract	Small-scale sillago, (Sillago parvisquamis) form the basis of a traditional recreational fishery in Japan. Historically distributed across a wide range, this species is now limited to a single population in the southwest region of the Suonada Sound in the Seto Inland Sea, Japan. Eleven candidate microsatellite loci were isolated from a small insert genomic DNA library of S. parvisquamis. In a screen of 40 individuals from Buzen Sea, we identified polymorphisms at all loci with levels of variability ranging from 2 to 20 alleles with no evidence of linkage disequilibrium. Observed heterozygosities ranged from 0.45 to 0.95, with no locus exhibiting significant departure from Hardy–Weinberg equilibrium. A test for cross-amplification with Sillago japonica revealed a subset of seven polymorphic loci that were successfully amplified. The polymorphic microsatellite loci developed in the current study will facilitate conservation genetic studies in both species, particularly the endangered S. parvisquamis.
Starting Page	771
Ending Page	773
Page Count	3
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	5
Issue Number	3
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-15
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sillago parvisquamis Endangered fish Microsatellite Conservation genetics Sillago japonica Conservation Biology/Ecology Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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