NDLI: Development of polymorphic chloroplast DNA markers for the endangered tree Eusideroxylon zwageri through chloroplast isolation and next-generation sequencing

Content Provider	Springer Nature Link
Author	Kurokochi, Hiroyuki Nurtjahjaningsih, I. L. G. Tan, Engkong Asakawa, Shuichi Saito, Yoko Ide, Yuji
Copyright Year	2015
Abstract	Borneo ironwood (Eusideroxylon zwageri) is a tall tropical tree that is threatened by illegal logging and exploitation due to its economic value. In the present study, approximately 10,618 bp of E. zwageri chloroplast DNA were sequenced genome-wide and 16 polymorphic markers of chloroplast DNA were developed, using next-generation sequencing technology. In total, 26 nucleotide substitutions, 3 indels, 1 sequence inversion, and 1 mononucleotide repeat variation were detected in samples of 10 trees collected from 5 populations in Kalimantan, Indonesia. These markers are a powerful tool that may be used to describe the phylogeographical genetic structure of E. zwageri and will be essential for the conservation and management of this endangered tree.
Starting Page	845
Ending Page	850
Page Count	6
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	7
Issue Number	4
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-08-22
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Eusideroxylon zwageri Next-generation sequencing Chloroplast DNA Polymorphic markers Phylogeography Conservation Biology/Ecology Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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