NDLI: Development of 35 novel microsatellite markers for the two-band anemonefish Amphiprion bicinctus

Content Provider	Springer Nature Link
Author	Nanninga, Gerrit Mughal, Mehreen Saenz Agudelo, Pablo Bayer, Till Berumen, Michael
Copyright Year	2012
Abstract	To investigate population genetic patterns of the anemonefish Amphiprion bicinctus in the Red Sea, we isolated and characterized 35 microsatellite loci using 454-sequencing. Microsatellite sequences were identified using the Tandem Repeats Finder program. The 35 loci were tested on 80 individuals sampled from two spatially separated populations along the Saudi Arabian Red Sea coast. We found a mean of 10.9 alleles per locus and observed levels of heterozygosity ranged from 0.4 to 0.98. All loci were polymorphic, none deviated significantly from Hardy–Weinberg equilibrium, no linkage disequilibrium was observed and there was no evidence for null-alleles in both populations. The markers reported here constitute the first specific set for this species, and they are expected to contribute to future studies of connectivity in the Red Sea region.
Starting Page	515
Ending Page	518
Page Count	4
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	5
Issue Number	2
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-12-18
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Twoband clownfish Amphiprioninae Microsatellites Population genetics Conservation Biology/Ecology Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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