NDLI: Isolation and characterization of novel microsatellite DNA markers in the gilthead seabream (Sparus aurata)

Content Provider	Springer Nature Link
Author	Parati, K. Chavanne, H. Pozzi, A. Previtali, C. Cenadelli, S. Bongioni, G.
Copyright Year	2010
Abstract	The gilthead seabream (Sparus aurata) is one of the main marine fish species cultured in Europe. In order to enhance the efficiency of aquaculture production, there is an increasing interest to develop selective breeding programs and molecular markers especially useful for tracing pedigrees without having to rear families separately. Here we describe 12 new polymorphic microsatellites loci, whose polymorphism was evaluated in a sample of 32 unrelated individuals. For each locus, the number of alleles vary from 4 to 23 alleles with mean expected and observed heterozygosity of 0.71 and 0.67, respectively. Two out of the 12 loci tested (SA5-E06 and SA3-G06) showed significant deviation from HWE after Bonferroni correction (P < 0.0041), both with a large heterozygote deficiencies. None of the 12 loci showed significant linkage disequilibrium, following the Bonferroni correction (P > 0.05; adjusted P = 0.0008).These set of microsatellites could prove very effective for parentage inference and stock differentiation studies.
Starting Page	83
Ending Page	85
Page Count	3
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	3
Issue Number	1
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2010-08-09
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Gilthead seabream Microsatellites Sparus aurata Breeding programs Evolutionary Biology Animal Anatomy Morphology Histology Plant Sciences Conservation Biology/Ecology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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