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| Content Provider | Springer Nature Link |
|---|---|
| Author | Pinho, Catarina Rocha, Sara Carvalho, BruM. Lopes, Susana Mourão, Sofia Vallinoto, Marcelo Brunes, Tuliana O. Haddad, Célio F. B. Gonçalves, Helena Sequeira, Fernando Ferrand, Nuno |
| Copyright Year | 2009 |
| Abstract | We report new primers for the amplification and sequencing of 11 nuclear markers in squamate reptiles and anuran amphibians (five in squamates, six in anurans). Ten out of the 11 loci are introns (three of which are linked) that were amplified using an exon-primed, intron-crossing (EPIC) PCR strategy, whereas an eleventh locus spans part of a protein-coding gene. Squamate and anuran primers were initially developed for Lacerta schreiberi (Squamata: Lacertidae) and Pelodytes spp. (Anura: Pelodytidae), respectively. Cross-species amplification of the squamate markers was evaluated in four genera representing two additional families, whereas for anurans three genera corresponding to three additional families were tested. Three out of the five loci were successfully sequenced in all squamate taxa tested. Cross-amplification of the six anuran markers had lower, but still significant, success. We predict these markers will be of great utility for both population genetics and phylogenetic studies. |
| Starting Page | 181 |
| Ending Page | 185 |
| Page Count | 5 |
| File Format | |
| ISSN | 18777252 |
| Journal | Conservation Genetics Resources |
| Volume Number | 2 |
| Issue Number | 1 |
| e-ISSN | 18777260 |
| Language | English |
| Publisher | Springer Netherlands |
| Publisher Date | 2009-11-14 |
| Publisher Place | Dordrecht |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Anura Squamata Nuclear sequence markers Introns Evolutionary Biology Animal Anatomy Morphology Histology Plant Sciences Conservation Biology/Ecology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Ecology, Evolution, Behavior and Systematics |
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