NDLI: A suite of multiplexed microsatellite loci for the ground beetle Abax parallelepipedus (Piller and Mitterpacher, 1783) (Coleoptera, Carabidae)

Content Provider	Springer Nature Link
Author	Marcus, Tamar Assmann, Thorsten Durka, Walter Drees, Claudia
Copyright Year	2013
Abstract	We report two sets of polymorphic, multiplexed microsatellite markers for the ground beetle Abax parallelepipedus. As the species is flightless, restricted to forests and affected by habitat fragmentation it can serve as a model species for landscape and conservation genetics. A complete set of 20 loci can be amplified in five PCR reactions and sequenced in two rounds, and a subset of 14 loci can be analyzed together in one PCR run and one sequencing round. In a scan of 3,432 individuals from across Germany using the 14 loci subset, we found between three and 14 alleles per locus. After accounting for two loci that are apparently sex-linked, no significant deviations from Hardy–Weinberg equilibrium were found. None of the loci showed evidence for the presence of null alleles. No overall linkage disequilibrium was detected. Some of the loci can also be used to study other Abax species.
Starting Page	1151
Ending Page	1156
Page Count	6
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	5
Issue Number	4
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-06-13
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abax parallelepipedus Carabidae Landscape genetics Primers Conservation Biology/Ecology Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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