NDLI: Isolation and characterization of microsatellite loci in the Chinese water snake Enhydris chinensis (Colubridae)

Content Provider	Springer Nature Link
Author	Du, Yu Zheng, Xiao Xiao Yao, Yun Tao Lin, Chi Xian
Copyright Year	2014
Abstract	The Chinese water snake Enhydris chinensis is an ovoviviparous colubrid snake distributed in China and Vietnam. It is commonly used in the production of Chinese snake oil, which is known for treating ailments such as fever, joint pain, and headache. It is listed as a vulnerable species according to the China red data book of endangered animals. Specific molecular markers such as mitochondrial DNA and microsatellite loci are helpful for us to get a better understanding of population structure and evolutionary history of the snake and take effective conservation action for this species. Here, we characterize eight polymorphic microsatellite loci isolated from E. chinensis genomic libraries. Forty-seven individuals were collected from Ji’an population in Jiangxi, China. These markers revealed a high degree of genetic diversity (3–10 alleles per locus) and heterozygosity (H $_{O}$ ranged from 0.532 to 0.957, and H $_{E}$ ranged from 0.565 to 0.806). No locus exhibited significant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. The results of this paper will be useful for future study of population structure and genetic diversity of E. chinensis.
Starting Page	729
Ending Page	730
Page Count	2
File Format	PDF
ISSN	18777252
Journal	Conservation Genetics Resources
Volume Number	6
Issue Number	3
e-ISSN	18777260
Language	English
Publisher	Springer Netherlands
Publisher Date	2014-04-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Colubridae Snake Enhydris chinensis Microsatellites PCR primers Polymorphism Conservation Biology/Ecology Plant Sciences Animal Anatomy Morphology Histology Evolutionary Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Ecology, Evolution, Behavior and Systematics

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