NDLI: Altered Voltage Dependent Calcium Currents in a Neuronal Cell Line Derived From the Cerebral Cortex of a Trisomy 16 Fetal Mouse, an Animal Model of Down Syndrome

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Altered Voltage Dependent Calcium Currents in a Neuronal Cell Line Derived From the Cerebral Cortex of a Trisomy 16 Fetal Mouse, an Animal Model of Down Syndrome

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Altered Voltage Dependent Calcium Currents in a Neuronal Cell Line Derived From the Cerebral Cortex of a Trisomy 16 Fetal Mouse, an Animal Model of Down Syndrome

Content Provider	Springer Nature Link
Author	Acuña, Mario A. Pérez Nuñez, Ramón Noriega, Jorge Cárdenas, Ana María Bacigalupo, Juan Delgado, Ricardo Arriagada, Christian Segura Aguilar, Juan Caviedes, Raúl Caviedes, Pablo
Copyright Year	2011
Abstract	Human Down syndrome (DS) is determined by the trisomy of autosome 21 and is expressed by multiple abnormalities, being mental retardation the most striking feature. The condition results in altered electrical membrane properties (EMPs) of fetal neurons, which are qualitatively identical to those of trisomy 16 fetal mice (Ts16), an animal model of the human condition. Ts16 hippocampal cultured neurons reportedly exhibit increased voltage-dependent calcium currents (I $_{Ca}$) amplitude. Since Ts16 animals are unviable, we have established immortalized cell lines from the cerebral cortex of Ts16 (named CTb) and normal littermates (named CNh). Using the whole-cell patch-clamp technique, we have now studied I $_{Ca}$ in CTb and CNh cells. Current activation occurs at −40 mV in both cell lines (V $_{holding}$ = −80 mV). Trisomic cells exhibited a 2.4 fold increase in the maximal Ca$^{2+}$ current density compared to normal cells (CNh = −6.3 ± 0.77 pA/pF, n = 18; CTb = −16.4 ± 2.423 pA/pF; P < 0.01, n = 13). Time dependent kinetics for activation and inactivation did not differ between the two cell types. However, steady state inactivation studies revealed a 15 mV shift toward more depolarized potentials in the trisomic condition, suggesting that altered voltage dependence of inactivation may underlie the increased current density. Further, the total charge movement across the membrane is increased in CTb cells, in agreement with that expected by the potential sensitivity shift. These results indicate that CTb cells present altered Ca$^{2+}$ currents, similar to those of Ts16 primary cultured central neurons. The CTb cell line represents a model for studying DS-related impairments of EMPs.
Starting Page	59
Ending Page	68
Page Count	10
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	22
Issue Number	1
e-ISSN	14763524
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-12-28
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Down syndrome Calcium currents Trisomy Patch clamp Neurology Neurochemistry Neurobiology Pharmacology/Toxicology Cell Biology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

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