NDLI: Human Umbilical Cord Blood-Derived Neural Stem Cell Line as a Screening Model for Toxicity

Content Provider	Springer Nature Link
Author	Patnaik, Rajashree Padhy, Rabindra Nath
Copyright Year	2016
Abstract	The aim was to investigate whether a human neural stem cell (NSC) line derived from human umbilical cord blood (hUCB) can be used for toxicity study. Toxicity of both neurotoxic environmental xenobiotics, methyl mercury chloride (CH$_{3}$HgCl), lead acetate (CH$_{3}$COOPb), and chlorpyrifos (CP), and non-neurotoxic insecticide, dichlorvos, as well as non-neurotoxic drugs, theophylline and acetaminophen were assessed. Additionally, differentiation of neuronal and glial cell lines derived from hUCB was elucidated. It was observed that CH$_{3}$HgCl was more toxic to human NSCs in comparison to CH$_{3}$COOPb and CP. The minimum inhibitory concentration (MIC) value against NSCs was 3, 10, and 300 mg/L, in each staining process, acridine orange/ethidium bromide (AO/EB) staining, 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrazolium bromide (MTT) assay, and Hoechst staining, for CH$_{3}$HgCl, CP, and CH$_{3}$COOPb, respectively. CH$_{3}$HgCl had the LC$_{25}$ value as 10.0, 14.4, and 12.7 mg/L, by staining method mentioned in succession. CP had the LC$_{25}$ value as 21.9, 23.7, and 18.4 mg/L; similarly, CH$_{3}$COOPb had LC$_{25}$ values, successively as 616.9, 719.2, and 890.3 mg/L. LC$_{50}$ values ranged from 18.2 to 21.7 mg/L for CH$_{3}$HgCl, 56.4 to 60.2 mg/L for CP, and 1000 to 1460.1 for CH$_{3}$COOPb. Theophylline, acetaminophen, and dichlorvos had no impact on the viability of NSCs. This work justified that hUCB-NSC model can be used for toxicity study.
Starting Page	319
Ending Page	326
Page Count	8
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	31
Issue Number	3
e-ISSN	14763524
Language	English
Publisher	Springer US
Publisher Date	2016-11-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neural stem cell Immunocytochemistry Cytolethality Umbilical cord blood Neurosciences Neurology Neurochemistry Pharmacology/Toxicology Neurobiology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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