NDLI: Nanocapsules with Polyelectrolyte Shell as a Platform for 1,25-dihydroxyvitamin D3 Neuroprotection: Study in Organotypic Hippocampal Slices

Content Provider	Springer Nature Link
Author	Ślusarczyk, Joanna Piotrowski, Marek Szczepanowicz, Krzysztof Regulska, Magdalena Leśkiewicz, Monika Warszyński, Piotr Budziszewska, Bogusława Lasoń, Władysław Basta Kaim, Agnieszka
Copyright Year	2016
Abstract	Calcitriol (1,25-dihydroxyvitamin D3), an active metabolite of vitamin D3, besides the role in calcium and phosphorus metabolism, plays a role in maintaining the functions of the brain. Active forms of vitamin D3 stimulate neurotrophic factors’ expression, regulate brain immune processes, and prevent neuronal damage. Therefore, a potential utility of vitamin D3 in a therapy of neurodegenerative disorders should be taken into account. On the other hand, systemic vitamin D3 treatment carries the risk of undesirable effects, e.g., hypercalcemia. Thus, 1,25-dihydroxyvitamin D3 targeting delivery by nanoparticles would be a tremendous advancement in treatment of brain disorders. Calcitriol was enclosed in emulsion-templated nanocapsules with different polymeric shells: PLL (Poly(l-lysine hydrobromide)), PLL/PGA (/Poly(l-glutamic acid)), and PLL/PGA-g-PEG (Poly(l-glutamic acid) grafted with polyethylene glycol). The average size of all synthesized nanocapsules ranged from −80 to −100 nm. Biocompatibilities of synthesized nanocarriers were examined in hippocampal organotypic cultures in basal conditions and after treatment with lipopolysaccharide (LPS) using various biochemical tests. We demonstrated that nanocapsules coated with PLL were toxic, while PLL/PGA- and PLL/PGA-g-PEG-covered ones were nontoxic and used for further experiments. Our study demonstrated that in LPS-treated hippocampal slices, both types of loaded nanoparticles have protective ability. Our findings underlined that the neuroprotective action of vitamin D3 in both free and nanoparticle forms seems to be related to the suppression of LPS-induced nitric oxide release.
Starting Page	581
Ending Page	592
Page Count	12
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	30
Issue Number	4
e-ISSN	14763524
Language	English
Publisher	Springer US
Publisher Date	2016-07-15
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Nanocapsules 1,25-dihydroxyvitamin D3 Encapsulation Polyelectrolytes Hippocampal organotypic cultures Neuroprotection Neurodegeneration Nitric oxide Neurosciences Neurology Neurochemistry Pharmacology/Toxicology Neurobiology Cell Biology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

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