NDLI: Methylene blue prevents neurodegeneration caused by rotenone in the retina

Content Provider	Springer Nature Link
Author	Zhang, Xian C. Rojas, Julio Gonzalez Lima, F.
Copyright Year	2006
Abstract	An experimental optic neuropathy model was used to test the hypothesis that methylene blue may protect the retinal ganglion cell layer from neurodegeneration caused by rotenone. Rotenone is a widely used pesticide that inhibits complex I, the first enzyme of the mitochondrial respiratory chain. Complex I dysfunction is linked to the degeneration of retinal ganglion cells in Leber’s optic neuropathy. Methylene blue is a reduction-oxidation agent that can act as a powerful antioxidant and also as an enhancer of the electron transport chain, preventing formation of mitochondrial oxygen free radicals and promoting oxygen consumption. The neurodegeneration of the retina was studied in mice with intravitreal microinjection of rotenone alone, or in combination with increasing doses of methylene blue, in one eye, and the vehicle in the contralateral control eye. The effect of rotenone and rotenone plus methylene blue was investigated using two histological stains, complex I and Nissl, and two measurements, morphometric layer thickness and non-biased stereological cell counts. Rotenone induced neurodegeneration in the retinal ganglion cell layer 24 h after injection, as indicated by significant reductions in both the thickness and cell numbers of the retinal ganglion cell layer of eyes microinjected with rotenone as compared to the control eyes. This neurodegeneration was prevented in a dose dependent manner by the injection of methylene blue along with rotenone. It was concluded that rotenone-induced degeneration in the ganglion cell layer can be prevented by intravitreal injection of methylene blue.In vitro experiments showed that methylene blue is both a powerful antioxidant as well as an enhancer of cellular oxygen consumption and is able to reverse the oxidative stress and decrease in oxygen consumption induced by rotenone in brain homogenates. The findings suggest that methylene blue may be a promising neuroprotective agent in optic neuropathy and perhaps other neurodegenerative diseases caused by mitochondrial dysfunction.
Starting Page	47
Ending Page	57
Page Count	11
File Format	PDF
ISSN	10298428
Journal	Neurotoxicity Research
Volume Number	9
Issue Number	1
e-ISSN	14763524
Language	English
Publisher	Springer-Verlag
Publisher Date	2006-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Methylene blue Rotenone Neurodegeneration Neuroprotection Optic neuropathy Neurosciences Pharmaceutical Sciences/Technology Neurology Neurochemistry Pharmacology/Toxicology Neurobiology
Content Type	Text
Resource Type	Article
Subject	Neuroscience Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Methylene Blue Provides Behavioral and Metabolic Neuroprotection Against Optic Neuropathy

Valproic Acid is Neuroprotective in the Rotenone Rat Model of Parkinson’s Disease: Involvement of α-Synuclein

Neuroprotection in Parkinson’s disease; a commentary

Gene-environment interplay in neurogenesis and neurodegeneration

Salmonella lipopolysaccharide (LPS) mediated neurodegeneration in hippocampal slice cultures

The putative neuroprotective role of dopamine agonists in parkinson’s disease

Rotenone produces opposite effects upon mouse striatal dopamine function as a result of environmental temperature

Neuroprotection against Aβ and glutamate toxicity by melatonin: Are GABA receptors involved?

Degree of damage compensation by various pacap treatments in monosodium glutamate-induced retinal degeneration

Methylene blue prevents neurodegeneration caused by rotenone in the retina

Similar Documents

Methylene Blue Provides Behavioral and Metabolic Neuroprotection Against Optic Neuropathy

Valproic Acid is Neuroprotective in the Rotenone Rat Model of Parkinson’s Disease: Involvement of α-Synuclein

Neuroprotection in Parkinson’s disease; a commentary

Gene-environment interplay in neurogenesis and neurodegeneration

Salmonella lipopolysaccharide (LPS) mediated neurodegeneration in hippocampal slice cultures

The putative neuroprotective role of dopamine agonists in parkinson’s disease

Rotenone produces opposite effects upon mouse striatal dopamine function as a result of environmental temperature

Neuroprotection against Aβ and glutamate toxicity by melatonin: Are GABA receptors involved?

Degree of damage compensation by various pacap treatments in monosodium glutamate-induced retinal degeneration

Methylene blue prevents neurodegeneration caused by rotenone in the retina