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Kinetics of the enzymatic hydrolysis of lignocellulosic materials at different concentrations of the substrate

Content Provider	Springer Nature Link
Author	Budaeva, V. V. Skiba, E. A. Baibakova, O. V. Makarova, E. I. Orlov, S. E. Kukhlenko, A. A. Udoratina, E. V. Shcherbakova, T. P. Kuchin, A. V. Sakovich, G. V.
Copyright Year	2016
Abstract	The kinetics of the enzymatic hydrolysis of two substrates—lignocellulosic materials from Miscanthus and oat hulls—in an acetate buffer is studied at different concentrations of the substrates. The substrates are obtained via single-step treatment with a dilute solution of nitric acid. The content of a nonhydrolyzable component—acid-insoluble lignin—for Miscanthus and oat hulls was 11 and 14%, respectively. A multi-enzyme composition of commercially available enzyme preparations CelloLux-A and BrewZyme BGX was used as a catalyst. It is shown that treatment with the nitric acid solution produces reactive substrates for the enzymatic hydrolysis. The innovative science of the results is confirmed by Russian patent 2533921. Kinetics of the enzymatic hydrolysis of these substrates in an acetate buffer can be described by a mathematical model based on a modified Michaelis–Menten equation. The main kinetic constants for both substrates are determined from the experimental data. The equilibrium concentrations of reducing substances (RSes) for the substrates are calculated from the initial substrate concentrations. It is found that within the studied range of substrate concentrations (33.3–120.0 g/L), the initial rate of enzymatic hydrolysis for the lignocellulosic material from oat hulls is higher than that for the lignocellulosic material from Miscanthus by 1 g/(L h). It is shown that the yield of RS depends of the initial concentration of the substrates: as the concentration rises from 33.3 to 120 g/L, the yield of RS falls 1.5–2.0 times, due to substrate inhibition. At low initial concentrations, the yields of RS are similar for the substrates from Miscanthus and oat hulls. When the initial concentration of the substrate reaches 120 g/L, the yield of reducing substances for the lignocellulosic material from Miscanthus is approximately 20% higher than that for the lignocellulosic material from oat hulls. The established dependences and the proposed mathematical model allow us to optimize the initial concentration of the substrate for efficient enzymatic hydrolysis.
Starting Page	81
Ending Page	87
Page Count	7
File Format	PDF
ISSN	20700504
Journal	Catalysis in Industry
Volume Number	8
Issue Number	1
e-ISSN	20700555
Language	English
Publisher	Pleiades Publishing
Publisher Date	2016-04-28
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	oat hulls Miscanthus substrate concentrations kinetics of enzymatic hydrolysis mathematical model Catalysis
Content Type	Text
Resource Type	Article
Subject	Catalysis

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