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Simple and rapid detection of rice false smut pathogen Ustilaginoidea virens in rice seeds

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Simple and rapid detection of rice false smut pathogen Ustilaginoidea virens in rice seeds

Content Provider	Springer Nature Link
Author	Chen, Yu Yao, Jian Li, Yun Fei Wang, Wen Xiang Yang, Xue Zhang, Ai Fang
Copyright Year	2013
Abstract	A method for simple and rapid detection of Ustilaginoidea virens in rice seeds was developed based on specific polymerase chain reaction (PCR). To design the specific primers for detection of U. virens, the comparison was made on 5.8S rDNA intra- and inter-specific variations in nucleotide sequences of U. virens and other pathogens: Fusarium verticillioides, Sclerotinia sclerotiorum, Tilletia barclayana, Fusarium graminearum and Magnaporthe oryzae. A 346-bp fragment could be amplified by using the specific primers uvr-F and uvr-R in U. virens, but not in other test fungi, indicating that the designed primers were specific for the detection of U. virens. U. virens was detected in nine of the 24 tested rice samples, indicating that 37.5% of the rice samples were contaminated with the false smut pathogen. The development of the simple and rapid detection technique using specific primers will be applicable for direct identification of U. virens in rice seeds to screen large numbers of samples.
Starting Page	371
Ending Page	375
Page Count	5
File Format	PDF
ISSN	03342123
Journal	Phytoparasitica
Volume Number	42
Issue Number	3
e-ISSN	18767184
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-12-20
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oryza sativa Polymerase chain reaction Villosiclava virens Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Insect Science

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