NDLI: Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR

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Phytoparasitica : Volume 45, Issue 4, September 2017

Diversity of the cultivable gut bacterial communities associated with the fruit flies Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae)

Parasitism of Psytallia concolor (Hymenoptera: Braconidae) on Bactrocera oleae (Diptera: Tephritidae) infesting different olive varieties

Effect of provision of apiaceous flowers associated to foods on the biology of Coleomegilla maculata

Impact of harvesting time on field carry over infestation of Sitophilus oryzae (L.) (Coleoptera: Curculionidae) in different maize genotypes

Insecticidal activity of essential oil from orange peels (Citrus sinensis) against Tribolium confusum, Callosobruchus maculatus and Sitophilus oryzae and its inhibitory effects on acetylcholinesterase and Na$^{+}$/K$^{+}$-ATPase activities

Plant oils as oviposition deterrents against winterform females of pear psylla, Cacopsylla pyri (L.) (Hemiptera: Psyllidae)

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Mode of inheritance of Indoxacarb resistance in diamondback moth, Plutella xylostella (L.) and cross resistance to different groups of pesticides

Induced defense dynamics in plant parts is requisite for resistance to Helicoverpa armigera (Hubner) infestation in chickpea

First report of the recently introduced, destructive powdery mildew Erysiphe corylacearum on hazelnut in Turkey

The application of lettuce in the qualitative and quantitative detection of Rhizoctonia Solani AG- 1 IA toxins

Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR

Effect of simultaneous and sequential inoculations of Meloidogyne incognita, Ralstonia solanacearum and Phomopsis vexans on eggplant in sand mix and fly ash mix soil

Best timing for glyphosate treatments and possible combinations with pre and post-emergence weed control practices in no-till maize

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Detection of Potato virus Y in industrial quantities of seed potatoes by TaqMan Real Time PCR

Content Provider	Springer Nature Link
Author	Avrahami Moyal, Liat Tam, Yehudit Brumin, Marina Prakash, Shanmugam Leibman, Diana Pearlsman, Malenia Bornstein, Menachem Sela, Noa Zeidan, Mouhammad Dar, Zion Zig, Uri Gal On, Amit Gaba, Victor
Copyright Year	2017
Abstract	Potato (Solanum tuberosum) is the largest crop in Israel. Production is based on the import of seed tubers from Europe for the spring crop. Imported tubers are generally free from virus infection. The most important virus infecting potato is Potato virus Y (PVY), which may cause severe damage to marketable yields. In Israel, tubers from the spring harvest are stored over the summer for planting in the autumn. It is important to be able to determine the infection rate of seed tuber lots from the spring harvest prior to storage. Commonly, infection is measured by sprouting tubers and measuring virus titre in the leaves using ELISA (the “Growing-On test”), which takes at least 6 weeks to give results. There is a need for a faster method to produce results, such as Taqman Real Time PCR (qPCR), for direct analysis of viral infection in tubers at harvest. To use qPCR as a diagnostic tool, it is necessary to demonstrate that both techniques give comparable results on batches of field-grown tubers. Such a comparison was performed on potential seed tuber lots of 14 different cultivars over three Israeli spring harvests (2013–2015). The agreement between the results of the two techniques was not of high statistical significance. However, the qPCR technique can distinguish well, by binary classification, between tuber lots with a low PVY infection rate (<5% by Growing On test; suitable for seed) and those unsuitable for seed (≥5% by Growing On test). Therefore, qPCR is an appropriate technique for determination of the PVY infection rate of seed tuber lots in Israel.
Starting Page	591
Ending Page	598
Page Count	8
File Format	PDF
ISSN	03342123
Journal	Phytoparasitica
Volume Number	45
Issue Number	4
e-ISSN	18767184
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-09-16
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Potato virus Y PVY Seed potatoes Real Time PCR Growing-On test Virus detection Plant Pathology Plant Sciences Ecology Agriculture Life Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science Insect Science

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