NDLI: Phylogenetic Analysis of Anopheles (Cellia) subpictus Grassi Using rDNA-ITS2 Sequence

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Proceedings of the Zoological Society : Volume 64

Proceedings of the Zoological Society : Volume 63

Proceedings of the Zoological Society : Volume 62

Phylogenetic Analysis of Anopheles (Cellia) subpictus Grassi Using rDNA-ITS2 Sequence

Content Provider	Springer Nature Link
Author	Chhilar, Jainder S. Chaudhry, Sudarshan
Copyright Year	2012
Abstract	Anopheles subpictus Grassi is one of the most abundant malaria vector mosquitos in Indian subcontinent especially in post monsoon months. This taxon has been speculated to be composed of four sibling species recognised using morphological and cytogenetic parameters provisionally named A, B, C and D. One of the sibling species ‘B’ has well documented status as a vector of malaria parasite in the Oriental region. Molecular phylogenetic analysis of this mosquito was done to discern sibling/cryptic species using internal transcribed spacer 2 region of the nuclear ribosomal DNA (rDNA-ITS2). An. culicifacies sibling species A, a member of Myzomia series, was used as an out-group to root the trees. In the present analysis rDNA-ITS2 region was PCR amplified, sequenced and the sequences obtained were then subjected to phylogenetic analysis with the sequences already present in the sequence database GenBank. Multiple sequence alignment was performed using ClustalX and further manually annotated in MEGA 4. Initial analysis suggested extreme 3′ end sequence divergence within different populations. Phylogenetic analysis of this spacer was done using maximum parsimony, maximum likelihood, and distance matrix—neighbour joining methods performed with bootstrapped dataset that were executed in DNAPARS, DNAML, DNADIST and neighbour programs in Phylip software package respectively. Phylogenetic trees produced were similar in topology but differed in bootstrap support. Results support the division of this taxon into at least two major clades differing in their sequence composition and product size that are recommended to be renamed as An. subpictus inland form and An. subpictus coastal form. Differences include insertions/deletions, transitions and transversions; especially one large 3′ end deletion event. The sibling species status of An. subpictus has been analysed critically.
Starting Page	1
Ending Page	10
Page Count	10
File Format	PDF
ISSN	03735893
Journal	Proceedings of the Zoological Society
Volume Number	65
Issue Number	1
e-ISSN	09746919
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-04-11
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Anopheles subpictus rDNA-ITS2 Phylogeny Sibling species Conservation Biology/Ecology Animal Genetics and Genomics Biodiversity Life Sciences Animal Anatomy Morphology Histology Zoology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Aquatic Science Immunology Animal Science and Zoology Ecology, Evolution, Behavior and Systematics Endocrinology Insect Science

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