NDLI: Water proton spin-lattice relaxation time during the apoptotic process in ultraviolet-irradiated murine erythroleukemia cells

Content Provider	Springer Nature Link
Author	Yamaguchi, Takeo Koga, Takayuki Katsuki, Satoshi
Copyright Year	2009
Abstract	Ultraviolet (UV) light-induced apoptosis has been extensively examined, but data on properties of intracellular water are insufficient. Thus, we examined the $^{1}$H spin-lattice relaxation time (T1) of intracellular water during apoptosis in murine erythroleukemia (MEL) cells. Values of T1 decreased upon cell shrinkage, whereas increased upon cell swelling. Similar results were obtained in MEL cells exposed to osmotic stress. Furthermore, the increment of T1 values, ultrastructure loss on cell surface, and DNA fragmentation in UV-treated cells were significantly suppressed by pan-caspase inhibitor. Taken together, these results suggest that the T1 of intracellular water can be used to estimate the relative content of bound and free water during UV-induced apoptosis in MEL cells.
Starting Page	131
Ending Page	136
Page Count	6
File Format	PDF
ISSN	18806546
Journal	The Journal of Physiological Sciences
Volume Number	59
Issue Number	2
e-ISSN	18806562
Language	English
Publisher	Springer Japan
Publisher Date	2009-01-22
Publisher Place	Japan
Access Restriction	Subscribed
Subject Keyword	Apoptosis Spin-lattice relaxation time Ultraviolet light Water flux Neurobiology Cell Physiology Animal Physiology Animal Biochemistry Neurosciences Human Physiology
Content Type	Text
Resource Type	Article
Subject	Physiology

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