NDLI: Guanine-cytosine contents of the host and symbiont cDNA in a symbiotic coral

Content Provider	Springer Nature Link
Author	Kii, Shin Ichi Tanaka, Jiro Watanabe, Toshiki
Copyright Year	2007
Abstract	Hermatypic (reef-building) corals harbor dinoflagellate endo-symbionts Symbiodinium spp. In studying gene expression in such symbiotic corals, problems arise regarding how to distinguish the coral and symbiont mRNA, and how to estimate their fractions in the mRNA population of the holobiont (symbiotic complex of the coral and Symbiodinium cells). In this study, these issues were addressed using juveniles of hermatypic coral Acropora tenuis in symbiosis with Symbiodinium cells of strain PL-TS-1. First, the guanine-cytosine (GC) contents were determined in expressed sequence tags (EST) from PL-TS-1 cells cultured in vitro and symbiont-free larvae of A. tenuis, and their average GC contents were found to be significantly different. The average GC content of the EST from the holobiont was much closer to that of A. tenuis larvae, suggesting that the majority (>90%) of mRNA isolated from the holobiont originated in the host. In protein-coding sequences, little overlap was observed between the GC-content distributions of PL-TS-1 cells and A. tenuis larvae. All of the coding sequences (n=59) found in the A. tenuis EST had GC contents below 0.5, whereas the GC content exceeded 0.5 in the majority (43/44) of coding sequences from the nuclear genome of PL-TS-1 cells.
Starting Page	1362
Ending Page	1372
Page Count	11
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	73
Issue Number	6
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cnidaria coral dinoflagellate GC content gene expression scleractinia Symbiodinium symbiosis Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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