NDLI: Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius

Content Provider	Springer Nature Link
Author	Okashita, Yuki Wang, Heng Tsue, Shiori Nishimiya, Osamu Ura, Kazuhiro Takagi, Yasuaki
Copyright Year	2017
Abstract	In the present study, we examined the localization of the major yolk protein (MYP) in the intestine of the sea urchin Strongylocentrotus intermedius. First, partial MYP complementary DNA was isolated from the sea urchin intestine. The expression level of MYP messenger RNA (mRNA) along the sea urchin digestive tract is highest in the intestine, so we performed in situ hybridization and immunohistochemical analysis using this tissue. No MYP mRNA was detected in the luminal epithelium, connective tissue, muscle tissue, or coelomic epithelium by in situ hybridization analysis. Positive immunohistochemical staining was observed in the luminal epithelium, inner epithelium and connective tissue, the signal being strongest in the latter. We conclude that MYP synthesized in the inner epithelial cells is moved to and stored in connective tissue and the luminal epithelium, before being secreted into the body cavity and the inner digestive cavity of the sea urchin.
Starting Page	803
Ending Page	810
Page Count	8
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	83
Issue Number	5
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2017-07-25
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Sea urchin Major yolk protein Immunohistochemistry In situ hybridization Intestine Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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The major yolk protein in sea urchin egg yolk granules is a glycoprotein complex

Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius

Purification and biochemical characterization of a cellulase from the digestive organs of the short-spined sea urchin Strongylocentrotus intermedius

Avian predation on wild and cultured sea urchin Strongylocentrotus intermedius in a rocky shore habitat

Feeding of the short-spined sea urchin Strongylocentrotus intermedius on macroalgae and benthic animals

Population structure of purple sea urchin Strongylocentrotus purpuratus along the Baja California peninsula

Effect of low temperature rearing, using deep-sea water, on gonadal maturation of the short-spined sea urchin, Strongylocentrotus intermedius, in Rausu, Hokkaido

Effects of biofilms as the main and as a supplementary food on the survival, somatic growth and gonad enhancement of sea urchin Strongylocentrotus intermedius

Effects of inbreeding on growth, gametogenesis, gonad production, quality and MYP expression in the sea urchin Strongylocentrotus intermedius

Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius

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Localization of major yolk protein in the digestive tract of the sea urchin Strongylocentrotus intermedius