NDLI: Expression of gonadotropin genes in Manchurian trout Brachymystax lenok and production of recombinant gonadotropins

Content Provider	Springer Nature Link
Author	Choi, Eunju Ko, Hyeyeon Shin, Jihye Kim, Mi Ae Sohn, Young Chang
Copyright Year	2005
Abstract	Manchurian trout Brachymystax lenok is an endangered fish species in East Asia including the Korean peninsula. To establish a method for artificial propagation and to improve our understanding of reproduction in the species, cloning of the pituitary gonadotropin (GTH) subunit cDNAs encoding the Manchurian trout gonadotropin common α (mtGTHα), follicle-stimulating hormone β (mtFSHβ), and luteinizing hormone β (mtLHβ) by polymerase chain reaction, were attempted. The open reading frames of the mtGTHα, mtFSHβ and mtLHβ encoded 114, 137, and 142 amino acid residues, respectively. The mature peptides showed strikingly high sequence identities to those of salmonid species GTH α2, FSHβ, and LHβ subunits (92–100%). In an examination of tissue distribution of the mtGTHα, mtFSHβ, and mtLHβ, it was revealed that all of the three subunit genes are specifically expressed in the pituitary by reverse transcription-polymerase chain reaction analysis. Next, single-chain recombinant FSH and LH constructs using the mtGTHα, mtFSHβ, and mtLHβ cDNAs were produced. In a transient transfection of the FSH and LH constructs into Chinese hamster ovary (CHO-K1) cells followed by Western blot analysis, the tethered FSH in cell lysates or LH secreted into a medium was detected from 48 to 72 h after transfection. The present study provides a possibility of bioactive GTH production for studies on the reproductive physiology of GTH in the Manchurian trout.
Starting Page	1193
Ending Page	1200
Page Count	8
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	71
Issue Number	6
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	cDNA CHO cell gonadotropins Manchurian trout recombinant hormone RT-PCR Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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