NDLI: Autolysis of krill protein from North Pacific krill Euphausia pacifica during protein recovery via isoelectric solubilization/precipitation

Content Provider	Springer Nature Link
Author	Sun, Le Chang Chen, Yong Lin Zhong, Chan Okazaki, Emiko Cao, Min Jie Weng, Wu Yin Osako, Kazufumi
Copyright Year	2014
Abstract	Autolysis of North Pacific krill protein in acidic and alkaline solubilization during protein recovery via isoelectric solubilization/precipitation was investigated. Pronounced auto-degradation of myosin heavy chain and actin from krill protein was observed at alkaline pH values (pH 9.0–12.0), with maximum autolytic activity at about pH 12.0. Meanwhile, proteolytic activity in krill protein at pH 12.0 was observed at low temperatures, suggesting a possible cause for the autolysis of krill protein during the protein recovery process. Three major proteinases were detected by zymographic analysis of myofibrillar protein, with approximate molecular weights of 26 kDa (KP-1), 18 kDa (KP-2) and 17 kDa (KP-3). KP-3 was active over a pH range from pH 5.0–12.0, suggesting that it may be responsible for the autolysis of krill protein during alkaline-induced protein recovery. Further study on substrate specificity and inhibitory specificity of KP-3 showed that KP-3 is serine type proteinase.
Starting Page	839
Ending Page	847
Page Count	9
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	80
Issue Number	4
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2014-05-06
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Autolysis Krill protein Isoelectric solubilization/precipitation Zymography Myofibrillar protein Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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