NDLI: Isolation of ligament protein-1 from shell ligament of the scallop Patinopecten yessoensis

Content Provider	Springer Nature Link
Author	Li, Ruihua Takahashi, Kohji Hori, Tomohiko Torita, Akane Hasegawa, Yasushi
Copyright Year	2012
Abstract	Elastomeric proteins occur in the shell ligament of bivalve molluscan shellfish. A newly identified protein named ligament protein-1, with a molecular mass of approximately 12 kDa, was extracted from the shell ligament of the scallop, Patinopecten yessoensis, with 70 % formic acid, and purified by gel filtration column chromatography and reverse-phase C18 column chromatography. Although the amino acid composition of this protein differs from that of the known shell ligament protein abductin, the partial amino acid sequences are similar to those of abductin. CD spectra and FT–IR spectra analyses showed that ligament protein-1 retains its secondary structures of α-helix, β-turn, and β-sheets. To the best of our knowledge, this is the first study on protein purification from the shell ligament of a bivalve molluscan shell.
Starting Page	1315
Ending Page	1320
Page Count	6
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	78
Issue Number	6
e-ISSN	14442906
Language	English
Publisher	Springer Japan
Publisher Date	2012-09-29
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Shell ligament Protein purification Scallop shell Food Science Freshwater & Marine Ecology Fish & Wildlife Biology & Management
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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Isolation of ligament protein-1 from shell ligament of the scallop Patinopecten yessoensis